We narrowed to 2,230 results for: Kap

1. His-TEV-PRC1

   Plasmid

   #167539

   Purpose

   For expression of His6-TEV-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK372

   Plasmid

   #73597

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-15 fatty acid desaturase
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK370

   Plasmid

   #73595

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 2)
   

   Use

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Silent mutation in Arg299 codon.

   Promoter

   GAL1 and GAL10

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAM9

   Plasmid

   #78560

   Purpose

   pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.

   Depositor

   James Kaper

   Article

   Merrell et al J Bacteriol. 2001 May;183(9):2746-54.

   Insert

   ompU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCVD468

   Plasmid

   #66155

   Purpose

   expression of His-tagged espA

   Depositor

   James Kaper

   Article

   Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

   Insert

   espA
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pJK057

   Plasmid

   #75036

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes residues 2-7 to His (SSRNNP>HHHHHH)

   Promoter

   T7 and n/a

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVD469

   Plasmid

   #66156

   Purpose

   expression of His-tagged espB

   Depositor

   James Kaper

   Article

   Karpman et al Pediatr Nephrol. 2002 Mar;17(3):201-11.

   Insert

   espB
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pJK350

   Plasmid

   #71560

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala365>Asp mutant (H6AaAlr-A365D)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   mutation changes alanine-365 to aspartate

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEST-PT7extended-MGapt-UTR1-deGFP

   Plasmid

   #227646

   Purpose

   pT7-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis with extended T7 promoter.

   Depositor

   Richard Murray

   Article

   Kapasiawala et al bioRxiv 2023.03.22.533877

   Insert

   MG aptamer (MGapt) and deGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDK362

    Plasmid

    #134194

    Purpose

    Retroviral plasmid with SKAP insert

    Depositor

    Iain Cheeseman

    Insert

    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    aa 166-316 with G5 and Jens siRNA hardening

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK606

    Plasmid

    #72529

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK566

    Plasmid

    #71586

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) (YfdE)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK290

    Plasmid

    #71554

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with double mutant (AaAlr-K39A,Y260A)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes lysine-39 to alanine and tyrosine-260 to …

    Promoter

    T7

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK326

    Plasmid

    #71557

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368>Asp mutant (H6AaAlr-A368D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-368 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK325

    Plasmid

    #71556

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367>Asp mutant (H6AaAlr-A367D)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes alanine-367 to aspartate

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK500

    Plasmid

    #72245

    Purpose

    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and endogenous

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK345

    Plasmid

    #71559

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala367/Ala368 deletion (H6AaAlr-A367del,A368del)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletes alanine-367 and alanine-368

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK344

    Plasmid

    #71558

    Purpose

    Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag and Ala368 deletion (H6AaAlr-A368del)

    Depositor

    T. J. Kappock

    Article

    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

    Insert

    alanine racemase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    deletes alanine-367 (same as deleting alanine-368)

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK624

    Plasmid

    #72539

    Purpose

    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK625

    Plasmid

    #72541

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge