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Showing: 581 - 600 of 2901 results

  4. pFLAG-WTS-1

    Plasmid
    #66856
    Purpose
    Express FLAG-tagged C.elegans WTS-1 in mammalian cells
    Depositor
    Yutaka Hata
    Article
    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.
    Insert
    WTS-1 (wts-1 Nematode)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E153G and D871G mutations in WTS1 compared to Gen…
    Promoter
    CMV
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pHW582

    Plasmid
    #198819
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescence
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
    Expression
    Worm
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198819-plasmid-map-sequence-id-390987

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  9. pHW539

    Plasmid
    #198811
    Purpose
    Dominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
    Expression
    Worm
    Mutation
    G310D mutation to abolish the binding of cAMP of …
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198811-plasmid-map-sequence-id-391700

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  10. pD002

    Plasmid
    #198833
    Purpose
    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::miniSOG-103L-mCherry::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198833-plasmid-map-sequence-id-391659

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  11. pHW580

    Plasmid
    #198816
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    198816-plasmid-map-sequence-id-390989

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  14. KG#84

    Plasmid
    #110878
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110878-plasmid-map-sequence-id-217951

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  15. KG#81

    Plasmid
    #110876
    Purpose
    Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscle
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    myo-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110876-plasmid-map-sequence-id-212571

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  16. KG#83

    Plasmid
    #110877
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    rab-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110877-plasmid-map-sequence-id-212517

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  17. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  18. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  19. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163095-plasmid-map-sequence-id-320590

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Showing: 581 - 600 of 2901 results