We narrowed to 705 results for: gc

1. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234446

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234439

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234440

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234450

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1-SA-CAG-Pa01-BFP

   Plasmid

   #234142

   Purpose

   Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   Pa01
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Donor-attp-u6-blank-sgrna-backbone

   Plasmid

   #234076

   Purpose

   Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

   Depositor

   Gary Hon

   Article

   Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

   Insert

   sgRNA-Blank
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234449

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YjdC-R82A_pET-14b

   Plasmid

   #233276

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 82…

   Promoter

   T7 promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YjdC-R64A_pET-14b

   Plasmid

   #233263

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

   Depositor

   Mohan Babu, Andrew Emili

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 64…

   Promoter

   T7 promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RICS

    Plasmid

    #25208

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RICS:E18-F218 (ARHGAP32 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pNHA_OROV_NSm

    Plasmid

    #208935

    Purpose

    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSm
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 949 bp from 5' end (Gn), deleted 29…

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SARS-CoV-2 S HexaPro

    Plasmid

    #154754

    Purpose

    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

    Depositor

    Jason McLellan

    Article

    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

    Insert

    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234453

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-pcDNA3.1 Puro-CAG-Voltron2

    Plasmid

    #172909

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234454

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV; Flp/frt

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Rosa26-LSL-PE2 Targeting Vector

    Plasmid

    #196038

    Purpose

    The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    Cas9-MMLV-RT
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HemH-R212A_pET-14b

    Plasmid

    #233262

    Purpose

    Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

    Depositor

    Mohan Babu, Andrew Emili

    Article

    Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

    Insert

    HemH (hemH )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Arginine was replaced with Alanine at position 21…

    Promoter

    T7 promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHA_OROV_NSm

    Plasmid

    #208940

    Purpose

    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSm
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 949 bp from 5' end (Gn), deleted 29…

    Promoter

    CMV

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid

    #172910

    Purpose

    Mammalian expression of voltage sensor; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    Voltron2-ST
    

    Use

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

    Plasmid

    #180486

    Purpose

    Mammalian expression of voltage sensor

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Insert

    VARNAM A122D
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    CAG

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge