We narrowed to 1,242 results for: membrane gfp

1. PMT-mEGFP_2

   Plasmid

   #203778

   Purpose

   Brightness calibration

   Depositor

   Thorsten Wohland

   Article

   Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

   Insert

   PMT-mEGFP dimer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR SFFV GLP1R(S301A)-EGFP

   Plasmid

   #158123

   Purpose

   Lentiviral plasmid for expression of GLP1R(S301)-EGFP fusion with SFFV promoter.

   Depositor

   Neal Devaraj

   Insert

   GLP1R (GLP1R )
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Serine 301 changed to alanine to enhance membrane…

   Promoter

   SFFV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP Cav2.2 human HAgHA-plus exon 18a - Short-Cterm - Arginine -in pcDNA3

   Plasmid

   #198915

   Purpose

   N-terminal GFP tagged, exofacial HAgHA tag in Domain II, contains exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

   Depositor

   Annette Dolphin

   Article

   Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

   Insert

   Cav2.2 (CACNA1B Human)
   

   Use

   Tags

   N-terminal GFP, internal double HA

   Expression

   Mammalian

   Mutation

   snp rs2278973 Arginine

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP Cav2.2 HA L702P pcDNA3

   Plasmid

   #206056

   Purpose

   expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

   Depositor

   Annette Dolphin

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L702P

   Promoter

   CMV

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP Cav2.2 HA D665A pcDNA3

   Plasmid

   #206113

   Purpose

   expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional

   Depositor

   Annette Dolphin

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D665A

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Venus-7aa-Cb5-pEGFP-C1

   Plasmid

   #166764

   Purpose

   To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5a Rat)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP Cav2.2 HA L702 P pCAGGS

   Plasmid

   #206055

   Purpose

   expression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional

   Depositor

   Annette Dolphin

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   L702P

   Promoter

   CMV/B-actin

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCerulean3-Bcl2-ActA-pEGFP-C1

   Plasmid

   #177418

   Purpose

   To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-2-ActA, Bcl2-acta, Bcl-acta
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCerulean3-Bcl2-cb5-pEGFP-C1

   Plasmid

   #177421

   Purpose

   Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).

   Depositor

   David Andrews

   Insert

   Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. myr-palm-mCherry2-mEGFP

    Plasmid

    #182859

    Purpose

    Hetero-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mCherry2-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. myr-palm-mEYFP-(L)-mEGFP

    Plasmid

    #182852

    Purpose

    Hetero-dimer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mEYFP-mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

    Plasmid

    #180311

    Purpose

    bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-myrGFP

    Plasmid

    #115502

    Purpose

    expression of myristoylated, plasma membrane targeted GFP

    Depositor

    Thomas Biederer

    Article

    Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

    Insert

    myristoylated GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

15. GFP Cav2.2 human HAgHA -minus exon 18a -LONG-Cterm in pcDNA3

    Plasmid

    #198914

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a - Long form of alternatively spliced C-teminus, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid

    #177427

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycines 153 and 154 mutated to alanine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid

    #177428

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Glycine 154 replaced with STOP codon

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP Cav2.2 human -HAgHA-minus exon 18a -Short-Cterm - Arg -in pcDNA3

    Plasmid

    #198916

    Purpose

    N-terminal GFP tagged, exofacial HAgHA tag in Domain II, does not contain exon 18a, Short form of alternatively spliced C-teminus, SNP rs2278973 variant Arginine, in pcDNA3 vector

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Function (Oxf). 2023 Oct 19;5(1):zqad060

    Insert

    Cav2.2 (CACNA1B Human)
    

    Use

    Tags

    N-terminal GFP, internal double HA

    Expression

    Mammalian

    Mutation

    exon 18a deleted, exon 19 starts 3 nucleotides ea…

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tet-myr-GFP

    Plasmid

    #83468

    Purpose

    Lentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Myristoylated GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline-inducible promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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