We narrowed to 20,434 results for: ACE

Showing: 6001 - 6020 of 20434 results

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

Plasmid

#111925

Purpose

Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CanlonicSF

Plasmid

#178342

Purpose

To explore lactate dynamics in intact systems

Depositor

Alejandro San Martín

Article

Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

Insert

CanlonicSF

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHPHS853

Plasmid

#228236

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
EYFP

Tags

HA and PuroR

Expression

Mammalian

Promoter

TRE3GV and none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.1

Plasmid

#218822

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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amilCP chromoprotein promoter-less

Plasmid

#118008

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, amilCP

Use

Synthetic Biology; Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBa.M6PR-GFP

Plasmid

#224407

Purpose

Mammalian expression of M6PR

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

M6PR (M6PR Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

doublecortin (DCX Human)

Tags

TurboID and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

Plasmid

#111923

Purpose

Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_19μ

Tags

CoxVIIIx2

Expression

Mammalian

Mutation

E67D, F92W, E104D, D133E at CaM

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

Plasmid

#70191

Purpose

Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

ClpX (clpX )

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG

Plasmid

#217737

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-846

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG

Plasmid

#217740

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL2 (ADGRL2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-830

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pR-mTagBFP2

Plasmid

#228596

Purpose

Mammalian expression of mTagBFP2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTagBFP2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mRuby3

Plasmid

#228609

Purpose

Mammalian expression of mRuby3, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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