We narrowed to 15,703 results for: jun
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Plasmid#208689PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h pan-neuronally in zebrafishDepositorInsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseCloning vectorPromoterHucAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMG81-MBP-t-M.MpeI-N374K-His
Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
TagsHis and MBPExpressionBacterialMutationM.MpeI-N374KAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-BAFFR-CFP
Plasmid#187002PurposeExpression of human BAFFR-CFP fusion proteinDepositorAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
3322_pETcon_SARS2_Omicron-BA2
Plasmid#184409Purposeyeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBDDepositorInsertSARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationG339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351
Plasmid#170198PurposeMammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)DepositorInsertSARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
TagsHis6ExpressionMammalianMutationL18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…PromoterCMV51Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mClover3-CD4-bla
Plasmid#179448PurposeDonor vector to knock in mClover3 N-terminal to human PER2 geneDepositorInsertmClover3
UseCRISPRTagsHis/FlagPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G
Plasmid#170201PurposeMammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)DepositorInsertSARS-CoV-2 S (spike) D614G (S SARS-CoV-2)
TagsHis6ExpressionMammalianMutationD614GPromoterCMV51Available sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -0
Plasmid#180010PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.1.7
Plasmid#171747PurposeMammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)DepositorInsertSpike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
TagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available sinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
P-rTetO-mCherry-T2A-RPL22-1xV5
Plasmid#170325PurposeTetracycline-inducible expression of V5 and mCherry tagged RPL22DepositorAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
Plasmid#157986PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Loop2-8A8G
Plasmid#157983PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Loop2-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-STOP-TdT
Plasmid#126425PurposeExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)DepositorInsertCas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
ExpressionMammalianMutationalanine-6 of TdT has been changed to a STOP codonPromoterpCMVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LSB-hsa-miR-221-3p
Plasmid#103365PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-3p target (MIR221 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-221-5p
Plasmid#103366PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-221-5p target (MIR221 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only