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pLv-HSA-uDys/eGFP

Plasmid

#26810

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

dystrophin (DMD Human)

Use

Lentiviral; Lentiviral plasmid backbone from l. n…

Tags

eGFP

Expression

Mutation

deleted R4-R23 deleted exons 71-78

Promoter

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet

Plasmid

#169219

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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prhaBAD-pA-Hia5

Plasmid

#222303

Purpose

Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

pA-Hia5

Use

Tags

6His and protein A

Expression

Bacterial

Mutation

codon optimized for bacterial expression

Promoter

rhaBAD

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-cMyc

Plasmid

#46970

Purpose

lentiviral expression of c-Myc

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC_P2A_Hygro_Barcode

Plasmid

#120461

Purpose

Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Use

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGD-γb

Plasmid

#196327

Purpose

35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

barley stripe mosaic virus γb

Use

Tags

Expression

Plant

Mutation

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-GINKO2

Plasmid

#177116

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

GINKO2

Use

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Use

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
Twinkle-APEX2-V5

Plasmid

#129705

Purpose

APEX2 fused to the mitochondrial nucleoid protein Twinkle

Depositor

Alice Ting

Article

Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

Insert

Twinkle-APEX2-V5 (TWNK, APEX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 TMEM192-Flag-FKBP

Plasmid

#112743

Purpose

express TMEM192-Flag-FKBP

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

TMEM192-Flag-FKBP (TMEM192 Human)

Use

Lentiviral

Tags

Flag, FKBP

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D770-N771 insNPG

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Use

Tags

smGdP-10XOllas

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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EGFR (del3) L747-E749del, A750P

Plasmid

#11015

Purpose

Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (del3) (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L747-E749 deleted, A750P

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only