We narrowed to 6,351 results for: Rel

1. psigma2-EGFP

   Plasmid

   #53610

   Purpose

   Expresses sigma2 of adaptor protein of AP2

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   sigma2 (Ap2s1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG-CMV10-hAtg13

   Plasmid

   #22872

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pJRoC30_Th3.14

   Plasmid

   #188068

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-Rab8a-QL

   Plasmid

   #86076

   Purpose

   A mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed glutamine 67 to leucine

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Rab8a-TN

   Plasmid

   #86077

   Purpose

   A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed threonine 22 to aspargine

   Promoter

   CMV

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

   Plasmid

   #199197

   Purpose

   Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int-BlastR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

   Plasmid

   #199198

   Purpose

   Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int-G418R
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RXFP1-DuET

   Plasmid

   #213373

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   RXFP1 (RXFP1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

   Plasmid

   #199196

   Purpose

   Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCI-neo-hApg5(K130R)-HA

    Plasmid

    #22949

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

    Insert

    Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    130 Lysine to Arginine

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pET21-Drp1

    Plasmid

    #72927

    Purpose

    Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

    Depositor

    David Chan

    Article

    Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

    Insert

    Drp1 (Dnm1l Mouse)
    

    Use

    Tags

    6xHis-tag and PreScission Protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. KHBD00476

    Plasmid

    #39585

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5838 (Dref Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

    Plasmid

    #203803

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q68L

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDO001

    Plasmid

    #198828

    Purpose

    Inhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNAIIIB rac1 QL

    Plasmid

    #61633

    Purpose

    mammalian expression of rac1 constitutively active mutant

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q61L, constitutively active

    Promoter

    CMV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pSG5-HA-ERR3 S57,81,219A

    Plasmid

    #37850

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    Estrogen-related receptor gamma (Esrrg Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Serines 57, 81, and 219 to Alanine (AGC t…

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only