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pLeo-A-GEMS(JAK-STAT)

Plasmid

#209109

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-A-a3-GEMS(JAK-STAT)

Plasmid

#209111

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor A-a3-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

A-a3-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-B-a2-GEMS(JAK-STAT)

Plasmid

#209112

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor B-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

B-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLeo-G-a2-GEMS(JAK-STAT)

Plasmid

#209113

Purpose

Transient mammalian expression of the CC functionalized GEMS receptor G-a2-GEMS (JAK-STAT)

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

G-a2-GEMS(JAK-STAT)

Expression

Mammalian

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

Plasmid

#195054

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pLDP1

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

Plasmid

#195053

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pFBA

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

Plasmid

#195052

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pXYL

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

Plasmid

#195051

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pGPD

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

Plasmid

#195050

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pADH2

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1L

Plasmid

#209568

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1S

Plasmid

#209569

Purpose

Mammalian expression of Shtn1 short isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 short isoform (4930506M07Rik Mouse)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BR1-P2A-FusionRed

Plasmid

#192819

Purpose

Expresses BR1 with FusionRed tag in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

BR1 (BRI1 Mustard Weed)

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (b)

Plasmid

#204429

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E626A T630A K668A E671A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-2

Plasmid

#204433

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

K614A H615A D835A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Palpha6 and Interface-4 (a)

Plasmid

#204434

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

F860A Y861A Y862A Y863A Q864A R865A H866A F867A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-9

Plasmid

#204440

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

E213A Q217A R557A D565A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W61*F

Plasmid

#198442

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F, R95*A,

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W5F

Plasmid

#198436

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W5F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W83F

Plasmid

#198439

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W83F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gZiPro W83F

Plasmid

#198446

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W83F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W69F

Plasmid

#198438

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W69F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W89F

Plasmid

#198440

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W89F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gZiPro W69F

Plasmid

#198445

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W69F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro

Plasmid

#198434

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eZiPro W61*F

Plasmid

#198435

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge