We narrowed to 32,142 results for: eng

1. pET-pro-siRNA-V2-LMNA

   Plasmid

   #111088

   Purpose

   pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

   Depositor

   Linfeng HUANG

   Article

   Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

   Insert

   Lamin A/C (LMNA Human)
   

   Expression

   Bacterial

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80065

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2-TBG-FLAG-mRIPK1-S321A

   Plasmid

   #115342

   Purpose

   Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

   Depositor

   Junying Yuan

   Article

   Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

   Insert

   RIPK1 (Ripk1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S321A

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG-CMV-Drosha (NLS-P426)

   Plasmid

   #171538

   Purpose

   Encodes mutant human Drosha protein

   Depositor

   Yan Zeng

   Article

   Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

   Insert

   DROSHA (DROSHA Human)
   

   Expression

   Mammalian

   Mutation

   an SV40 NLS sequence (PKKKRKVGI) insertion into t…

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79889

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT-myl7-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80061

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

   Plasmid

   #89890

   Purpose

   BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   HA-BirA-2A-citrine
   

   Use

   Unspecified

   Tags

   Citrine

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTB2-RanGAP-Cerulean-Avi

   Plasmid

   #80068

   Purpose

   Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
   

   Use

   Zebrafish biotagging

   Tags

   Avi

   Expression

   Bacterial

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

   Plasmid

   #62978

   Purpose

   Expresses myc-tagged mouse βTrCP residues 292-569

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   BTRC (Btrc Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   codes for residues 292-569

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAWp28-v1 scaffold

    Plasmid

    #157976

    Purpose

    pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. human ETV6 gRNA-4

    Plasmid

    #133404

    Purpose

    human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV2-TBG-FLAG-mRIPK1-S321E

    Plasmid

    #115343

    Purpose

    Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-GRP78-C73 (pGEX4T1)

    Plasmid

    #238428

    Purpose

    Expresses GRP78 with the only the last 73 amino acids at the C terminus fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-GRP78-Delta C17 (pGEX4T1)

    Plasmid

    #238426

    Purpose

    Expresses GRP78 with the last 17 amino acids at the C terminus deleted fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-GRP78-Delta C11(pGEX4T1)

    Plasmid

    #238425

    Purpose

    Expresses GRP78 with the last 11 amino acids at the C terminus deleted fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-GRP78-Delta C73 (pGEX4T1)

    Plasmid

    #238427

    Purpose

    Expresses GRP78 with the last 73 amino acids at the C terminus deleted fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-GRP78-Delta KDEL (pGEX4T1)

    Plasmid

    #238424

    Purpose

    Expresses GRP78 Delta KDEL fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX4T-1 PDZD8-CT

    Plasmid

    #227854

    Purpose

    To express in E. coli PDZD8-CT

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD44v-EC-His (pcDNA3)

    Plasmid

    #238418

    Purpose

    Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

    Insert

    CD44 containing extracellular region of CD44v (CD44 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-TNT4-Zfr-P2A-HA-luciferase

    Plasmid

    #223188

    Purpose

    Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Insert

    luciferase (LOC116160065 firefly)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Promoter

    TNNT2

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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