We narrowed to 10,337 results for: plasmids 101

1. pSNRW-Z3-Nluc

   Plasmid

   #174486

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z3-Nluc
   

   Use

   Tags

   6x Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EED-KO-gRNA-2

   Plasmid

   #131322

   Purpose

   gRNA to knockout EED. Use with EED-KO-gRNA-1

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   EED-KO-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

3. KG#493

   Plasmid

   #110932

   Purpose

   Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

   Depositor

   Kenneth Miller

   Article

   Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

   Inserts

   unc-17 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. KG#94

   Plasmid

   #110931

   Purpose

   Expresses proteins in C. elegans cholinergic neurons in head, ventral nerve cord, and tail

   Depositor

   Kenneth Miller

   Article

   Williams et al Genes Dev. 2007 Nov 1;21(21):2731-46. do

   Inserts

   unc-17 promoter
   unc-54 3' control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet30b-5M SrtA

   Plasmid

   #51140

   Purpose

   expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Article

   Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

   Insert

   S.aureus SrtA 5M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #102796

   Purpose

   Retrovirus for delivery of one sgRNA (empty, bbsI)

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   U6 sgRNA
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcCas12nHS_RV6_empty

   Plasmid

   #203811

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiV_Neo_LKB1

   Plasmid

   #108111

   Purpose

   Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   LKB1 (STK11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS promoter

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_SIK3

    Plasmid

    #108103

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiUGE smFP-V5 donor ORF2

    Plasmid

    #200382

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHiUGE smFP-V5 donor ORF0

    Plasmid

    #200380

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHiUGE smFP-V5 donor ORF1

    Plasmid

    #200381

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tetO7_Ef1a_SOX2

    Plasmid

    #18919

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only