We narrowed to 22,590 results for: promoter

1. lentiCRISPR - Amplicon, JAK2 sgRNA 1

   Plasmid

   #70679

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFIN-mIRBP156-CHER-RK-GFP-WPRE

   Plasmid

   #44593

   Depositor

   Susan Semple-Rowland

   Article

   Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

   Inserts

   IRBP156-CHER
   RK-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human rhodopsin kinase (pos 3723-4019) and murine…

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB-puro-mU6-CNPsgRNA

   Plasmid

   #126610

   Purpose

   Expresses CNP sgRNA under mouse U6 promoter

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   CNP sgRNA (Cnp Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFAB3913

   Plasmid

   #47816

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   see insert

   Available since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CDK4-luciferase active (880)

   Plasmid

   #86657

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   867 base pairs including up to coding nucleotide …

   Promoter

   CDK5

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-vsv-Incenp wt-GFP

   Plasmid

   #108502

   Purpose

   inducible expression of vsv-INCENP wt-EGFP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124544

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41 C55A, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124540

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124538

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-1 (GB1204)

    Plasmid

    #75405

    Purpose

    Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    AtU6-1 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid

    #70660

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

    Plasmid

    #61792

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhag 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pSLQ1371-sgITGB1-3

    Plasmid

    #121534

    Purpose

    sgITGB1-3 sequence: GTTCAGTGAATGGGAACAACG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB1-3
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Use

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Hmga2 ATG wt

    Plasmid

    #52034

    Purpose

    encodes wt HMGA2

    Depositor

    Julian Downward

    Article

    Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    starting ATG deleted

    Promoter

    CMV

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMM549

    Plasmid

    #112531

    Purpose

    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HCKan-P-TEF2

    Plasmid

    #65337

    Purpose

    construction of TEF2 promoter in HCKan-P receiving vector

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FgH1tUTG_huMcl-1.2

    Plasmid

    #85531

    Purpose

    Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe-Neuroserpin oloop

    Plasmid

    #58260

    Purpose

    Retroviral vector for expressing mutated Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutant of neuroserpin lacking five residues from …

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. CDk4-luciferase fragment A

    Plasmid

    #86658

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    generated by cleavage at the NcoI site from 880 (…

    Promoter

    CDK6

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge