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Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

Plasmid

#105196

Purpose

targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

Depositor

Peter Mombaerts

Article

Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

Insert

Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)

Use

Mouse Targeting

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only
AAV.CBA.YFP.miR-E_shRps6ka2-3

Plasmid

#180390

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-3 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-2

Plasmid

#180389

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-2 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-1

Plasmid

#180388

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-1 (Rps6ka2 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.KRAB-mArid3a.IRES.GFP

Plasmid

#169306

Purpose

Constitutive overexpression of murine KRAB-Arid3a fusion protein with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

KRAB-Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

SFFV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.VP64-mArid3a.IRES.GFP

Plasmid

#169313

Purpose

Constitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

VP64-Arid3a (Arid3a Mouse)

Use

Lentiviral

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh1

Plasmid

#168986

Purpose

Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9 (UBE2I )

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh3

Plasmid

#168985

Purpose

Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP APOBEC3 no UGI

Plasmid

#135352

Purpose

Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

rAPOBEC3-nSpyCas9 aa 1029

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 AIDx with UGI

Plasmid

#135354

Purpose

Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

hAIDx-nSpyCas9 aa-UGI

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 2

Plasmid

#135358

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

WT Tada-nSpyCas9 aa 1029-evolved Tada

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-66/myc-His

Plasmid

#127495

Purpose

Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-66 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-66

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 E57A/myc-His

Plasmid

#127497

Purpose

Expresses myc-tagged NDUFS2/E57A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 E57A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 E57A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 T58A/myc-His

Plasmid

#127498

Purpose

Expresses myc-tagged NDUFS2/T58A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 T58A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 T58A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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