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pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Staufen-dsRBD(+6)

Plasmid

#171546

Purpose

Encoding part of the Drosophila Staufen protein from M571 to P656

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Staufen (stau Fly)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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PW124-LMNA 5'HA-TriTag (mCherry)-3'HA (HDR donor)

Plasmid

#164047

Purpose

CRISPR donor plasmid to insert TriTag (mCherry) into the N-terminus of human LMNA gene

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

mCherry-TriTag

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-5U6-sgRNAs-hsyn-EGFP

Plasmid

#112213

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

sgRNAs

Use

AAV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIs8-4b-SaDAH::G226D

Plasmid

#140130

Purpose

Expresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase G226D variant from Sinomenium acutum

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Mutation

changed Gly226 to Asp226

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer-ETS-CORE-MUT

Plasmid

#138372

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

human 161K human enhancer with ETS bind sites mutated

Use

Gateway compatible

Mutation

ETS factor binding sites mutated

Promoter

NONE

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-KSR R615H

Plasmid

#118332

Purpose

Expression of mouse KSR

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2017 Jan 27;292(4):1449-146

Insert

KSR R615H (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

R615H

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX D144L

Plasmid

#111514

Purpose

Expresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Tags

His tag

Expression

Bacterial

Mutation

Aspartatic acid 144 to Leucine

Promoter

T7

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETC PITPbeta N89F

Plasmid

#58279

Purpose

bacterial expression of PITP beta N89F mutant

Depositor

Shamshad Cockcroft

Article

Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

N89F (abolishes PI binding)

Promoter

T7

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
NHA-Delta 12am

Plasmid

#36935

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 12am (TNRC6A Human)

Tags

NHA

Expression

Mammalian

Mutation

aa 896-1045 only; all GW or WG changed to AA

Available Since

May 30, 2012

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L25A

Plasmid

#30388

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L25A (PTEN Human)

Expression

Mammalian

Mutation

L25A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-D24N

Plasmid

#30387

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-D24N (PTEN Human)

Expression

Mammalian

Mutation

D24N

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L23F

Plasmid

#30386

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L23F (PTEN Human)

Expression

Mammalian

Mutation

L23F

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-F21A

Plasmid

#30385

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-F21A (PTEN Human)

Expression

Mammalian

Mutation

F21A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-R14A

Plasmid

#30384

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-R14A (PTEN Human)

Expression

Mammalian

Mutation

R14A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-R11A

Plasmid

#30383

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-R11A (PTEN Human)

Expression

Mammalian

Mutation

R11A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-K6A

Plasmid

#30382

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-K6A (PTEN Human)

Expression

Mammalian

Mutation

K6A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L186V

Plasmid

#30379

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L186V (PTEN Human)

Expression

Mammalian

Mutation

L186V

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only