We narrowed to 11,691 results for: NSI

1. pY001 (pFnCpf1_full)

   Plasmid

   #69973

   Purpose

   Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 locus
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Promoter

   myo6b

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER21-puro_TAOK3

   Plasmid

   #209778

   Purpose

   doxycycline-inducible expression of TAOK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK3 (TAOK3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-INU-FLAG-CCPG1

   Plasmid

   #128262

   Purpose

   Expresses CCPG1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   CCPG1 (CCPG1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdp43-EGFP construct4

   Plasmid

   #28197

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa216-414 only

   Available Since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

   Plasmid

   #100857

   Purpose

   AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1b
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

   Plasmid

   #164612

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Expression

   Mammalian

   Mutation

   S383T, F177A

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLADE(FP6**)-mCherry

    Plasmid

    #168049

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Expression

    Bacterial

    Promoter

    J23101** and PBAD

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_YES1-1/1

    Plasmid

    #91439

    Purpose

    Protein expression and purification of human SH3 domain construct YES1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    YES1-1/1 (YES1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 p53 S15A

    Plasmid

    #69004

    Purpose

    mammalian expression of human p53 S15A mutant.

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    S15A

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 Cyclin E1

    Plasmid

    #109348

    Purpose

    virus packaged, doxycyline inducible expression of Cyclin E1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cyclin E1 (CCNE1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Synapsin sHRPa-NRX in FSW lentiviral vector

    Plasmid

    #73147

    Purpose

    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-NRX
    

    Use

    Lentiviral

    Tags

    2x HA tag and Flag tag

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-ADAR2-sesRNA-GFP-W3SL

    Plasmid

    #192069

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Ctip2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC234r-ccdB/TPK2

    Plasmid

    #176179

    Purpose

    yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    dropout cassette containing toxic genes ccdB and TPK2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-JNKKTRClover

    Plasmid

    #59139

    Purpose

    ORF encoding JNK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-SspB-mScarlet-I-MYPT169

    Plasmid

    #178522

    Purpose

    Expresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    SspB-mScarlet-I-MYPT169
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIPZ-EGFP:LIMD1

    Plasmid

    #108230

    Purpose

    Lentiviral vector for Tet-inducible LIMD1 expression

    Depositor

    Kenneth Irvine

    Article

    Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Tet/ON Minimal CMV promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Tags

    3xFLAG

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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