We narrowed to 11,790 results for: Hal

1. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-CCND1-HA-puro

   Plasmid

   #212670

   Purpose

   Express tagged cyclin D1

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETconNK-TEM1(S70A,D179G)

   Plasmid

   #81166

   Purpose

   pETconNK with TEM1(S70A,D179G) beta-lactamase

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   GAL1 and gal1

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDL001_Hec1-ms_IgG_HC

   Plasmid

   #193618

   Purpose

   Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
   

   Use

   Tags

   His6 and signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDL002_Hec1-ms_IgG_LC

   Plasmid

   #193619

   Purpose

   Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 light chain (Hec1-ms_IgG_LC)
   

   Use

   Tags

   signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mOtop1_pGEMHE

   Plasmid

   #114674

   Purpose

   This construct was used to express mouse Otop1 in Xenopus laevis oocytes

   Depositor

   Emily Liman

   Article

   Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

   Insert

   Otopetrin 1 (Otop1 Mouse)
   

   Use

   For generating the mrna, which can highly express…

   Tags

   Expression

   Mutation

   SNP (rs49325632): Glycine 396 to Alanine

   Promoter

   T7

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY48

   Plasmid

   #191052

   Purpose

   klp-6 fluorescent neural reporter driving nuclear mNeptune2.5 and CyOFP1 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   klp-6 (klp-6 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEY47

   Plasmid

   #191051

   Purpose

   gcy-21 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   gcy-21 (gcy-21 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. optoPKAW196R/Y204A

    Plasmid

    #104546

    Purpose

    Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R/Y204A
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R and Y204A

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid

    #140222

    Purpose

    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    PGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE ENTR H1-miR-30 L1-R5

    Plasmid

    #62181

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    RNAi; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

13. p3E-APEX2-P2A-mKate2

    Plasmid

    #67671

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-P2A-mKate2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB556

    Plasmid

    #115966

    Purpose

    SGP insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 -98/15 SGP 2xTetApt-STOP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Clover-pBAD

    Plasmid

    #54575

    Purpose

    Localization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    6xHis and Clover

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPR-sgUBA5

    Plasmid

    #86132

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA targeting UBA5 (UBA5 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0426 (CRISPR with spc3)

    Plasmid

    #130642

    Purpose

    Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    CRISPR(spacer-3)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY15

    Plasmid

    #191021

    Purpose

    flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    flp-1 (flp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. JW617

    Plasmid

    #64809

    Purpose

    CUC3 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain and Myc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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