We narrowed to 36,868 results for: kan
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Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)
Plasmid#160626PurposeTU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-dCas9:TV-Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOM-CD63-COM
Plasmid#138350PurposeExpressing COM-CD63-COM fusion protein in mammalian cellsDepositorInsertCOM-CD63-COM fusion protein
UseCRISPR and LentiviralTagsCOM is fused to the N and C termini of human CD63ExpressionMammalianAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163072PurposeEntry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseGateway CloningAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163075PurposeEntry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseGateway CloningAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-MAT2A
Plasmid#164820PurposeExpresses MAT2A in E. coli BL21(DE3)DepositorAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC1-CMV-DAAO-NES
Plasmid#141107PurposeExpresses DAAO with nuclear export signal in mammalian cellsDepositorInsertDAAO-NES
TagsNuclear export signalExpressionMammalianMutationdeleted DAAO amino acids 367-368PromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-AspS
Plasmid#159618PurposeExpression plasmid for AcpS phosphopantetheinyl transferaseDepositorInsertAcpS
Tags6HISExpressionBacterialAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
Tags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
B-MFF
Plasmid#141158PurposeDimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membraneDepositorAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR_Cas9_sp1
Plasmid#154851PurposeExpresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genomeDepositorInsertsSpcas9 gene
sgRNA scaffold
UseCRISPRTags6xHisMutationCodon harmonized variant for expression in Rhodob…Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDendra2-H3
Plasmid#157814PurposeFluorescent reporter for histone H3 trackingDepositorAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Akap1(31)-FRB-iRFP
Plasmid#139315Purposemitochondrial-resident FRB for chemically induced dimerizationDepositorInsertMus musculus Akap1(1-31) M16L:MTOR(2021-2113):iRFP
ExpressionMammalianMutationM16LAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-AmCyan
Plasmid#138481PurposeFor cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.DepositorInsertAmCyan
ExpressionMammalianAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
OSCA1.2
Plasmid#136593PurposeExpresses the mechanosensitive channel OSCA1.2 in mammalian cellsDepositorAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Hu_BIRC5(WT)
Plasmid#136351PurposeHuman BIRC5 cDNA in Gateway pENTRDepositorInsertBIRC5 (BIRC5 Human)
UseGateway CloningAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 19
Plasmid#134987PurposeExpression of human Myo 19 in mammalian cells.DepositorAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by