We narrowed to 10,047 results for: pET

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pHK302.5

Plasmid

#235470

Purpose

Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA5

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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WT USP7 UBL-12

Plasmid

#227230

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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I766T USP7 UBL-12

Plasmid

#227232

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al bioRxiv [Preprint]. 2026 Apr 28:2026.04.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

I766T; aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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L757P USP7 UBL-12

Plasmid

#227231

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Publication

Korchak et al bioRxiv [Preprint]. 2026 Apr 28:2026.04.

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

L757P; aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v10 bacteria

Plasmid

#208594

Purpose

Production and purification of catalytically active SynCas.v10

Depositor

Omar Akbari

Publication

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v10

Expression

Bacterial

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v10 bacteria

Plasmid

#208595

Purpose

Production and purification of catalytically inactive SynCas.v10

Depositor

Omar Akbari

Publication

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v10

Expression

Bacterial

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-SynCas.v12 bacteria

Plasmid

#208596

Purpose

Production and purification of catalytically active SynCas.v12

Depositor

Omar Akbari

Publication

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

SynCas.v12

Expression

Bacterial

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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T7-RBS-6xHis-SUMO-dSynCas.v12 bacteria

Plasmid

#208597

Purpose

Production and purification of catalytically inactive SynCas.v12

Depositor

Omar Akbari

Publication

Brogan et al J Mol Biol. 2026 Jan 15;438(2):169566. d

Insert

dSynCas.v12

Expression

Bacterial

Promoter

T7

Available since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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E67K SUMO1

Plasmid

#221065

Purpose

Expression of E67K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67K

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35K SUMO1

Plasmid

#221055

Purpose

Expression of H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35K

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35S SUMO1

Plasmid

#221056

Purpose

Expression of H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35S

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67R SUMO1

Plasmid

#221064

Purpose

Expression of E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67R

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35R H43R SUMO1

Plasmid

#221063

Purpose

Expression of H35R H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R H43R double mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35R, H43R

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H43R SUMO1

Plasmid

#221057

Purpose

Expression of H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43R

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H35R SUMO1

Plasmid

#221054

Purpose

Expression of H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35R

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H43K SUMO1

Plasmid

#221058

Purpose

Expression of H43K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43K

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H43S SUMO1

Plasmid

#221059

Purpose

Expression of H43S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H43S

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H75K SUMO1

Plasmid

#221061

Purpose

Expression of H75K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Publication

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H75K

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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