We narrowed to 1,307 results for: Cbs

1. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

   Plasmid

   #229827

   Purpose

   A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xnls-TagBFP2-MCS-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Puro-DEST (JDW 925)

   Plasmid

   #229808

   Purpose

   A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

   Plasmid

   #229811

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA transactivator-LexA-Operon-nls-GFP
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

   Plasmid

   #229817

   Purpose

   A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

   Plasmid

   #229819

   Purpose

   A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBeta-MHC-promoter DV (JDW 26)

   Plasmid

   #229835

   Purpose

   A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXMLC2-DEST (JDW 7)

   Plasmid

   #229833

   Purpose

   A gateway compatible destination vector containing the Xenopous myosin light chain 2 promoter for embryonic and adult cardiomyocyte expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-MCS (JDW 455)

    Plasmid

    #229832

    Purpose

    A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attL1/L2 flanked MCS
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGEN-DV-IRES-myr-BFP (JDW 491)

    Plasmid

    #229834

    Purpose

    A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-StayGoldc4-Giantin (JDW 1355)

    Plasmid

    #229844

    Purpose

    A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    n2StayGoldc4
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME nls BFP FLAG WPRE bGH pA (JDW 484)

    Plasmid

    #224539

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-mTagBFP2-FLAG_WPRE-bGH-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

    Plasmid

    #209411

    Purpose

    Expression of wild-type adult isoform of human SCN8A.

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

    Insert

    SCN8A Variant 3, stabilized (SCN8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Modifed human HSPA5 intron 4 inserted after bp237…

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-EF1a-pA (JDW 1222)

    Plasmid

    #224526

    Purpose

    A Gateway compatible 3' entry clone containing rat EF-1a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EF-1a polyA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-WPRE-bGH-pA (JDW 1221)

    Plasmid

    #224527

    Purpose

    A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-bGH
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

    Plasmid

    #224491

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-tdiRFP
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

    Plasmid

    #224524

    Purpose

    A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    rox-3xNLS-mCherry-V5-2xSTOP-rox
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p5E lexOP-c-fos min pro (JDW 1254)

    Plasmid

    #224477

    Purpose

    Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexOp
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p5E-hs_Cldn5 (JDW 1120)

    Plasmid

    #224481

    Purpose

    Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Claudin-5 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

    Plasmid

    #224486

    Purpose

    Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    H2B-V5-n2StayGoldC4
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pME V5 mScarlet-I (JDW 1323)

    Plasmid

    #224487

    Purpose

    Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

    Plasmid

    #224488

    Purpose

    Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pME-myr-mTagBFP2-Flag (JDW 1183)

    Plasmid

    #224492

    Purpose

    Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    myr-mTagBFP2-Flag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-Lifeact-mCherry-3xHA (JDW 1321)

    Plasmid

    #224493

    Purpose

    Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lifeact-mCherry-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge