We narrowed to 1,269 results for: MS2

1. pYPQ131D2.0

   Plasmid

   #100044

   Purpose

   Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   OsU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

   Plasmid

   #241137

   Purpose

   Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA

   Depositor

   Tim Stasevich

   Article

   Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

   Inserts

   Anti-GCN4 scFv
   tdMCP
   

   Tags

   HaloTag and sfGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCP-mCherry-TIS11B

   Plasmid

   #207670

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   TIS11B (ZFP36L1 Human)
   

   Tags

   MCP-mCherry

   Expression

   Mammalian

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. THI ONLY pALD4 T-

   Plasmid

   #161497

   Purpose

   Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

   Depositor

   Diethard Mattanovich, Brigitte Gasser

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2 (non activation control NAC)
   gRNA (Non targeting control NTC)
   

   Expression

   Yeast

   Promoter

   pALD4, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

   Plasmid

   #79380

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

   Plasmid

   #79379

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

   Plasmid

   #79382

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Chimera 4
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Tags

    mNeon

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQAT25

    Plasmid

    #223397

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid

    #164614

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.

    Depositor

    Jeffrey Chao

    Article

    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

    Insert

    stdMCP-stdHalo-RH1
    

    Use

    Lentiviral

    Tags

    HA tag and NLS

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG MCP-VP64

    Plasmid

    #92357

    Purpose

    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    MCP-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK389.AAV

    Plasmid

    #177327

    Purpose

    aTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Inserts

    SoxS
    dCas9
    

    Use

    CRISPR

    Tags

    MCP (MS2 Coat Protein)

    Mutation

    R93A,S101A

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCP-mCherry-SEC61B

    Plasmid

    #207671

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    SEC61B (SEC61B Human)
    

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241145

    Purpose

    Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    tdMCP - CAAX
    

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQAT26

    Plasmid

    #223398

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQAT28

    Plasmid

    #223400

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pYPQAT29

    Plasmid

    #223401

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYPQAT30

    Plasmid

    #223402

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MCP/PCP yellow RNA Lantern

    Plasmid

    #226096

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MCP/PCP red RNA Lantern

    Plasmid

    #226097

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid

    #241144

    Purpose

    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-GCN4 scFv
    tdMCP - CAAX
    

    Tags

    (n2)oxStayGold(c4) E147D and HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

    Plasmid

    #241139

    Purpose

    Expresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNA

    Depositor

    Tim Stasevich

    Article

    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

    Inserts

    Anti-HA frankenbody
    anti-GCN4 scfv
    tdMCP
    

    Tags

    HaloTag, mScarlet3, and sfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ141-ZmUbi-RZ-Aa3.8.4

    Plasmid

    #136375

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge