Addgene

Showing: 601 - 620 of 1561 results

Results per page:

Sort results by:

pDGB3_alpha1

Plasmid

#68228

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
wt1aCreERT2

Plasmid

#108464

Purpose

Used to generate a cryst:mCherry wt1a:CreERT2 zebrafish transgenic line.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

wt1aCreERT2

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPU-huTRIMmiR30-TRIM5

Plasmid

#132938

Purpose

All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPM-D4-miR30-TRIM5

Plasmid

#132932

Purpose

Lentivector for TRIM5 knockdown and puromycin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

TRIM5 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFP (GB0053)

Plasmid

#68197

Purpose

Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

YFP coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-Nanog-pA-pgk-hph

Plasmid

#74915

Purpose

Mammalian expression of mNanog

Depositor

Jose Silva

Article

Theunissen et al Curr Biol. 2011 Jan 11;21(1):65-71. doi:

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pABM-D4-miR30-CypA

Plasmid

#132934

Purpose

Lentivector for CypA knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

CypA shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_omega2

Plasmid

#68239

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPO640

Plasmid

#195296

Purpose

pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

miniMcCAST (Tn7575) with KanR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
iTol2Amp-γ-crystallin:venusGFP

Plasmid

#108462

Purpose

This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

iTol2Amp crystGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPU-huTRIMmiR30-L1221

Plasmid

#132937

Purpose

All-in-one huTRIM5 rescue-control shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRoCascade_gfp

Plasmid

#178771

Purpose

Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_omega1

Plasmid

#68238

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha2

Plasmid

#68229

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge