Addgene

We narrowed to 1,717 results for: FAST

Showing: 601 - 620 of 1717 results

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pcDNA5/FRT/TO_NES_STf

Plasmid

#182001

Purpose

CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

NES-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pZA72

Plasmid

#174783

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_XopJ4, Kmr plant selection marker

Use

Tags

Expression

Plant

Mutation

WT + STOP codon

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJS169

Plasmid

#48882

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

Insert

lacI with ssrA tag

Use

Synthetic Biology

Tags

ssrA (TSAANDENYALAA)

Expression

Bacterial

Mutation

Promoter

Para/lac

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-G_oT7pol-IRES-BSD

Plasmid

#172369

Purpose

Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oT7pol + BSD

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL11015

Plasmid

#117499

Purpose

FAST-Red selectable marker Golden Gate Level 1 Position 1

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI

Use

Tags

RFP

Expression

Plant

Mutation

Promoter

AtOLE1

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Use

Tags

fusion protein

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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rSytIV pIE

Plasmid

#12503

Depositor

Jane Sullivan

Article

Ting et al J Neurosci. 2006 Jan 11. 26(2):372-80.

Insert

Synaptotagmin IV (Syt4 Rat)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_STf

Plasmid

#182011

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lifeact_STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCoofy63

Plasmid

#122015

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His6-Smt3Star and Multi3: depending on the LP2 pr…

Expression

Insect

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2+pG10-90

Plasmid

#170879

Purpose

Phytobrick - pG10-90

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

pG10-90

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCoofy41

Plasmid

#55184

Purpose

Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Bacterial

Mutation

Promoter

pPH (Polyhedrin promoter)

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pOX + Shaker1a

Plasmid

#16139

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (ZK1321.2 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pCoofy42

Plasmid

#55190

Purpose

Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCoofy47

Plasmid

#55188

Purpose

Mammalian expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pZA57

Plasmid

#174770

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-4xMyc, Kmr plant selection marker

Use

Tags

4xMyc

Expression

Plant

Mutation

WT

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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