We narrowed to 2,669 results for: PGK
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Plasmid#191682PurposeDonor plasmid backbone PGK-puroRDepositorTypeEmpty backboneExpressionMammalianMutationWTAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only
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pLV-PGK-dt/CBA-EGFP-R106bT
Plasmid#163921PurposeServes as control to pLV-PGK-dt/CBA-EGFP-106bT.DepositorInsertdtomato and EGFP
UseLentiviralExpressionMammalianMutationAdded 4-tandem reverse orientation-mir-106b-targe…Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MKC177_CCR5(PGK-synEPOR)
Plasmid#232415PurposeAAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.DepositorAvailable SinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin
Plasmid#187949PurposeSMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertSMASh-SpdCas9-tagRFPt-P2A-tagBFP
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
ExpressionMammalianMutationD832APromoterPGKAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
EW215 PGK-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)
Plasmid#236130PurposePlasmid encoding the ZNF35-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF35(5-8)-deImmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW216 PGK-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236131PurposePlasmid encoding the ZNF250-derived portion of the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF250(1-4)-deImmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW418 PGK-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)
Plasmid#236155PurposePlasmid encoding the IKZF1-derived portion of the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertIKZF1(2-3)-deimmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187960PurposeAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1
Plasmid#187963PurposemAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.DepositorInsertsAID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
osTIR1
UseCRISPR and Synthetic BiologyTagsGGS linker and T2AExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EW209 PGK-ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF(FLP-IN)
Plasmid#236135PurposePlasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW196 PGK-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236132PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW415 PGK-ZNF35(5-8)-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)
Plasmid#236136PurposePlasmid encoding the ZNF35/ZNFIKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertZNF35/IKZF1(2-3)-deimmunLink-NZF
ExpressionMammalianPromoterPGKAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Venus-Akaluc
Plasmid#209768PurposeVenus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertVenus-Akaluc
UseLuciferase; Piggybac transposonExpressionMammalianPromoterCAGAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-FLAG-HA-CAD_WT-HA-Hygro
Plasmid#253792PurposepLenti lentiviral expression vector encoding FLAG- and HA-tagged human wild-type CAD under a human PGK promoter.DepositorAvailable SinceMay 1, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#196545PurposeDonor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorInsertSurface marker and Citrine reporter
UseSynthetic BiologyExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TV_KRT20-p2a-mScarlet-nls-p2a-icasp9_pgk-blast
Plasmid#251172PurposeTargeting vector for C-terminal knockin at KRT20 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9DepositorInsertp2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human KRT20 (KRT20 Human)
UseCRISPRTagsp2a-mScarlet-nls-p2a-icasp9Available SinceFeb. 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
TV_LGR5-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
Plasmid#251173PurposeTargeting vector for C-terminal knockin at LGR5 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTRDepositorInsertires-mNeon-nls-p2a-dtr-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human LGR5 (LGR5 Human)
UseCRISPRTagsires-mNeon-nls-p2a-dtrAvailable SinceFeb. 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V
Plasmid#187959PurposeExpressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.DepositorInsertsBlasticidin resistance
FKBP12_F36V
UseCRISPR and Synthetic BiologyTagsGGS linkerExpressionMammalianAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_TStrp_HA_Ct
Plasmid#194066Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsTwin_Strep-HAExpressionMammalianAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only