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Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCN38-yfp
Plasmid#227637PurposeNegative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-Empty Vector-IRES-Puro
Plasmid#120568PurposeFor cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]DepositorTypeEmpty backboneUseLentiviralTagsFlag-HA-6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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NUMB2-eGFP-M107K
Plasmid#37803DepositorInsertNUMB isoform 2 (NUMB Human)
UseLentiviralTagseGFPExpressionMutationPromoterpCMVAvailable sinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
UseTags6X Histidine and TEV cleavage siteExpressionYeastMutationPromoterADH1/yeastAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-dCas9VP192-T2A-EGFP
Plasmid#69536PurposeEpisomal plasmid encoding dCas9VP192DepositorInsertdCas9-dCas9VP192-EGFP
UseCRISPRTagsEGFPExpressionMutationPromoterCAGAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
NUMB4-eGFP-M107K
Plasmid#37805DepositorInsertNUMB isoform 4 (NUMB Human)
UseLentiviralTagseGFPExpressionMutationPromoterpCMVAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAF3 PHD_pGEX-6P-2
Plasmid#92100PurposeExpresses GST fusion TAF3 PHD domain proteinDepositorInsertTAF3 PHD domain (TAF3 Human)
UseTagsGST TagExpressionBacterialMutationPromotertac promoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP
Plasmid#183916PurposeReverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-eGFP-hTau P301L-WPRE
Plasmid#233043PurposeTo Express a GFP human Tau P301L fusion protein from a CMV promoterDepositorInserteGFP-hTau P301L fusion (MAPT Human, Synthetic)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Plasma membrane-targeted optoPLD
Plasmid#140114PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expressionDepositorInsertPhospholipase D
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
plvx-AD8
Plasmid#191558PurposeInducible expression of HIV-1 EnvDepositorInsertHIV-1 AD8 env
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFEC
Plasmid#67060PurposeStandard for cell-free expression benchmarking.DepositorInsertTFEC (TFEC Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-eGFP-hTau E14-WPRE
Plasmid#233042PurposeTo Express a GFP human Tau E14 fusion protein from a CMV promoterDepositorInserteGFP-hTau E14 fusion (MAPT Human, Synthetic)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NFIA1.1
Plasmid#112698PurposeExpression of NFIA1.1 protein for transient transfection assaysDepositorInsertNuclear Factor I A1.1 (Nfia Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-TREX1
Plasmid#164245Purposeretroviral plasmid for GFP-TREX1DepositorInsertTREX1 (TREX1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)
Plasmid#173454PurposeContains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-GFP-RPA70
Plasmid#164231Purposeretroviral plasmid for GFP-RPA70DepositorInsertRPA70 (RPA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only