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pZB604_pT7-sfGFP

Plasmid

#228510

Purpose

Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

sfGFP

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB641_pT7_Lysin-KU1

Plasmid

#228511

Purpose

T7 RNAP inducible Lysin KU1 Expression

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

Lysin KU1

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAM292

Plasmid

#226129

Purpose

Expression of His6-SUMO-Ub-mEos3.2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-intein-CBD

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM296

Plasmid

#226132

Purpose

Expression of Ufd1L55R-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1L55R-3C-His6

Expression

Bacterial

Mutation

L55R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM295

Plasmid

#226131

Purpose

Expression of Ufd1R64C-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1R64C-3C-His6

Expression

Bacterial

Mutation

R64C

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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EphA1-SAM-Cry2olig

Plasmid

#176600

Purpose

EphrinA1 RTK fusion to Cry2olig - includes SAM domain

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphrinA1-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pm-EphA1-SAM-Cry2olig

Plasmid

#176684

Purpose

membrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM domain

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

PM-EphA1-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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OgeuIscB ωRNA-v2

Plasmid

#220958

Purpose

human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Insert

OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-2-VP16

Plasmid

#213842

Purpose

Used for expressing VP16 fused to ZF-2.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 Full (1-261)

Plasmid

#99654

Purpose

Expresses dSp Cas9 fused to p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

p65 activation domain

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-C-PrLD

Tags

6xHis-tags

Expression

Bacterial

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRC043

Plasmid

#206799

Purpose

J23107.SoxS-GID1

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-GID1

Promoter

J23107

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRC025

Plasmid

#206796

Purpose

J23107.MCP-ABI

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-ABI

Promoter

J23107

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRC011

Plasmid

#206794

Purpose

J23107.MCP-SYNZIP6

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-SYNZIP

Promoter

J23107

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRC012

Plasmid

#206795

Purpose

J23107.SoxS-SYNZIP5

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-SYNZIP

Promoter

J23107

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRC027

Plasmid

#206797

Purpose

J23107.SoxS-PYL1

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

SoxS-PYL1

Promoter

J23107

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRC029

Plasmid

#206793

Purpose

J23107.MCP-SoxS

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-SoxS

Promoter

J23107

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRC042

Plasmid

#206798

Purpose

J23107.MCP-GAI

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

MCP-GAI

Promoter

J23107

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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FB430

Plasmid

#203621

Purpose

TU for the expression of phleomycin resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PpcbC:ble:TamdS

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB431

Plasmid

#203622

Purpose

TU for the expression of terbinafine resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PgpdA:ergA:TamdS

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pML32

Plasmid

#203344

Purpose

2-bit register circuit on pLW555 backbone: PTac-S2, PTet-P2, PLux-A1, PLux-H1

Depositor

Lauren Andrews

Article

Lebovich et al ACS Synth Biol. 2023 Aug 15. doi: 10.102

Inserts

srpR
phlF
amtR
hlyIIR

Promoter

PAmtR and PTet, PPhlF and PLux*, PSrpR and PLux*,…

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3

Plasmid

#203487

Purpose

Mitochondrial directed jAspSnFR3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Lentiviral

Tags

Mitochondrial localization signal

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-Green0029-35S

Plasmid

#193395

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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