We narrowed to 33,965 results for: CMV

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Ii-Str_neomycin

Plasmid

#65308

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-YFP

Plasmid

#55781

Purpose

This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

alpha-s-EE YFP (Gnas Rat)

Tags

EYFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

Met was substituted for Gln69 in EYFP (Clontech).…

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s(alpha3beta5/G226A/A366S)

Plasmid

#55797

Purpose

This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

Depositor

Catherine Berlot

Article

Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

Insert

alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(-)-Fc

Plasmid

#59313

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)

Tags

Fc (from human IgG)

Expression

Mammalian

Promoter

CMV, T7

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cyto-CaNARi

Plasmid

#64849

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, Nuclear export signal (NES), and Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NLS (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB R73Q

Plasmid

#60420

Purpose

Mammalian Expression of ITSN DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

hITSN 1159-1509 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mEos3.2-TOMM20-N-10

Plasmid

#57483

Purpose

Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Integrin-Beta1-N-18

Plasmid

#57390

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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EYFP-ER-0

Plasmid

#56589

Purpose

Localization: Endoplasmic Reticulum, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

ER (CALR Human)

Tags

EYFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
tdTomato-Golgi-7

Plasmid

#58100

Purpose

Localization: Golgi, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Golgi (B4GALT1 Human)

Tags

tdTomato

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
PEG-Actinin-C-sstFRET

Plasmid

#61101

Purpose

Expression of α-actinin with C-term sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Tags

sstFRET

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLX304-YAP1 _PDZ

Plasmid

#59147

Purpose

YAP1 mutants that harbored a deletion of the 5 amino acids

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PDZ binding domain (last 5aa) deleted

Promoter

CMV

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
EBFP2-Nucleus-7

Plasmid

#55249

Purpose

Localization: Nuclear Localization, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

SV40 T-Antigen Nuclear Localization Signal (NLS)

Tags

EBFP2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-Keratin-17

Plasmid

#55065

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Keratin (KRT18 Human)

Tags

mCherry

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Keratin14-N-18

Plasmid

#55066

Purpose

Localization: Cytokeratin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Keratin14 (KRT14 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
mEmerald-MyosinIIB-C-18

Plasmid

#54192

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

MyosinIIB (MYH10 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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