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Plasmid#105778PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsmClover3-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only
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pMEXneo-TrkB
Plasmid#190203PurposeTo express the Human TrkB receptor under the Moloney mouse sarcoma virus LTRDepositorInsertTrkB (NTRK2 Human)
UseTagsExpressionMammalianMutationPromoterMoloney mouse sarcoma virus right LTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPb LAP
Plasmid#66979PurposeExpression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of proteinDepositorInsertC/EBP beta (Cebpb Mouse)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgE/κ
Plasmid#61878PurposeHuman immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin Epsilon constant region
Human immunoglobulin kappa constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromotermEF1 Prom and rEF1 PromAvailable sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#1-mCherry_PGK-H2B-CFP
Plasmid#164103PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#1-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-HOXA9
Plasmid#97041PurposeExpresses HOXA9 in mammalian cellsDepositorInsertHOXA9 (HOXA9 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-dddAI
Plasmid#186559PurposeBroad host range regulated expression of the DddA immunity determinant (DddAI)DepositorInsertdddAI
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-RUNX1-P2A-ERG
Plasmid#97045PurposeExpresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertRunt Related Transcription Factor 1, ETS-Related Gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF1_P2A_Hygro_Barcode
Plasmid#120488PurposeBarcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertKLF1 (KLF1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
Plasmid#35509PurposeAAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorHas ServiceAAV5 and AAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#1-mVenus_PGK-H2B-CFP
Plasmid#164100PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-mVenus
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_CRX_P2A_Hygro_Barcode
Plasmid#120433PurposeBarcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertCRX (CRX Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-HA-ERK2 R67S
Plasmid#53201PurposeExpresses GST and HA tagged human ERK2 R67S from bacterial expression vectorDepositorInsertERK2 (MAPK1 Human)
UseTagsGST and HAExpressionBacterialMutationR67SPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
FH1-CLGT#2-mCherry-_PGK-H2B-CFP
Plasmid#164106PurposeFluorescent reporter for genetic tracing of classical Glioblastoma cell-state.DepositorInsertCLGT#2-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG-9.47_VP2_AS #2562
Plasmid#74238PurposeModified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.DepositorInsertsAAV2 Rep
AAV9.47 VP2-AS
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mTurquoise2
Plasmid#169222PurposeTargeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_LMNA
Plasmid#111688PurposeMAC-tagged gene expressionDepositorInsertLMNA (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2
Plasmid#169223PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HER3-DMVF-181-R
Plasmid#87621PurposeC-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.DepositorInsertHER3-DMVF181-C (ERBB3 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only