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pKK-mClover3-TEV

Plasmid

#105778

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mClover3-TEV

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXneo-TrkB

Plasmid

#190203

Purpose

To express the Human TrkB receptor under the Moloney mouse sarcoma virus LTR

Depositor

Pantelis Tsoulfas

Article

Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

Insert

TrkB (NTRK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Moloney mouse sarcoma virus right LTR

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPb LAP

Plasmid

#66979

Purpose

Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP beta (Cebpb Mouse)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pVITRO1-dV-IgE/κ

Plasmid

#61878

Purpose

Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin Epsilon constant region
Human immunoglobulin kappa constant region

Use

Tags

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

mEF1 Prom and rEF1 Prom

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#1-mCherry_PGK-H2B-CFP

Plasmid

#164103

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#1-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA9

Plasmid

#97041

Purpose

Expresses HOXA9 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

HOXA9 (HOXA9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-dddAI

Plasmid

#186559

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-3UAG

Plasmid

#82501

Purpose

Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescence Protein with 3 UAG codons

Use

Tags

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3, …

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF1_P2A_Hygro_Barcode

Plasmid

#120488

Purpose

Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF1 (KLF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#1-mVenus_PGK-H2B-CFP

Plasmid

#164100

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#1-mVenus

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_CRX_P2A_Hygro_Barcode

Plasmid

#120433

Purpose

Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CRX (CRX Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
FH1-CLGT#2-mCherry-_PGK-H2B-CFP

Plasmid

#164106

Purpose

Fluorescent reporter for genetic tracing of classical Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

CLGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP2_AS #2562

Plasmid

#74238

Purpose

Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP2-AS

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mTurquoise2

Plasmid

#169222

Purpose

Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_LMNA

Plasmid

#111688

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LMNA (LMNA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2

Plasmid

#169223

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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