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H2B-mScarlet3-S2

Plasmid

#246872

Purpose

Expression of mScarlet3-S2. This contruct is used for imaging, photobleaching and photochromic behavior measurements.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mScarlet3-S2-Lamin A/C

Plasmid

#246875

Purpose

Expression of mScarlet3-S2 fused with Lamin A/C. This construct is used for imaging.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-Y11

Plasmid

#239000

Purpose

Final circuit with -Ara: ON, +Ara: OFF behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression only in the absence of Ara (-Ara: ON, +Ara: OFF)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Plasmid

#238894

Purpose

EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.

Depositor

Gil Westmeyer

Article

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDR-pBAD-ATG-6xHIS-AsiA

Plasmid

#245835

Purpose

Express anti-sigma protein, AsiA, in E. coli. His-Tag for Western Blots or Purification.

Depositor

Jie Xiao

Article

Weng et al Proc Natl Acad Sci U S A. 2019 Oct 1;116

Insert

AsiA

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR myc-FUS-FUS-FusionRed-FUS-FRB-iLID

Plasmid

#223285

Purpose

A phase separating fluorescent protein with optogenetic and chemogenetic tags for recruitment of desired cargo domains. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

Myc-FUS-FUS-FusionRed-FUS-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pStayGold

Plasmid

#243674

Purpose

Expresses StayGold fluorescent protein in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

StayGold fluorescent protein

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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UMGIII

Plasmid

#245860

Purpose

Archael (Uncultured marine group III euryarchaeote clone 108exp4) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 October.

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1

Plasmid

#226819

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mRuby3

Plasmid

#244914

Purpose

cpSFGFP control with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mRuby3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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CARMEN

Plasmid

#240464

Purpose

Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER

Depositor

Roland Malli

Article

Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111

Insert

CARMEN

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSS22-IRES-NLS-eGFP

Plasmid

#241837

Purpose

Part I of the Donor construct for UBE3A reporter

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

eGFP

Use

Donor plasmid

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6423

Plasmid

#183860

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Incumbent Rescue A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6428

Plasmid

#183865

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Incumbent Rescue B regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6420

Plasmid

#183857

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Rescue Invader A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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JBL6421

Plasmid

#183858

Purpose

sfGFP reporter under the control of Mutant E. coli thiB TPP Riboswitch under the control of a Constitutive promoter

Depositor

Julius Lucks

Article

Berman et al RNA. 2023 Nov;29(11):1658-1672. doi: 10.

Insert

E. coli thiB TPP Riboswitch Full Rescue A regulated sfGFP

Expression

Bacterial

Promoter

J23119 - Anderson Promoter

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pETA_21 tRNA genes

Plasmid

#242431

Purpose

Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA032

Plasmid

#240781

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA031

Plasmid

#240780

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA030

Plasmid

#240779

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 pT7cons BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA023

Plasmid

#240772

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA022

Plasmid

#240771

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA021

Plasmid

#240770

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 Ptrc BCD_low - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA019

Plasmid

#240768

Purpose

Plasmid contains the triple selection cassette gfp -tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_medium - gfp -tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA018

Plasmid

#240767

Purpose

Plasmid contains the triple selection cassette gfp - tetApBR322-ROP- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA pBR322-ROP PrhaBAD BCD_low - gfp - tetApBR322-ROP- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA015

Plasmid

#240764

Purpose

Plasmid contains the triple selection cassette gfp - tetAp15A- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA p15A PrhaBAD BCD_low - gfp - tetAp15A- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SAGA014

Plasmid

#240763

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_high - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SAGA013

Plasmid

#240762

Purpose

Plasmid contains the triple selection cassette gfp - tetARK2- CmTrunc that allows for in vivo recombineering in E.coli.

Depositor

Morten Norholm

Article

Sepulchro et al Commun Biol. 2025 Jun 17;8(1):933. doi:

Insert

SAGA RK2 PrhaBAD BCD_medium - gfp - tetARK2- CmTrunc

Use

Synthetic Biology

Mutation

Mutation on translation initiation region of tetA

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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