We narrowed to 11,691 results for: NSI

1. pCI-neo-RL-ZEB2

   Plasmid

   #35536

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB2 gene (ZEB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SH3(2)-3R

   Plasmid

   #112091

   Purpose

   Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-3R (NCK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-ATP6V1D-HAGST

   Plasmid

   #87932

   Purpose

   C-term HA-GST tagged overexpression

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   ATP6V1D (ATP6V1D Human)
   

   Tags

   HA-GST

   Expression

   Mammalian

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3015b

   Plasmid

   #209317

   Purpose

   Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

   Depositor

   Thomas Hooven

   Article

   Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

   Insert

   Group B Streptococcus-compatible single guide RNA
   

   Expression

   Bacterial

   Promoter

   Xyl/tet promoter that is constitutively active

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV Flag PAI-RBP

   Plasmid

   #45452

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-JNKKTRmRuby2

   Plasmid

   #59148

   Purpose

   ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
   

   Tags

   mRuby2

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-RAB-EKARev-NLS

   Plasmid

   #118476

   Purpose

   ddRFP-based single-color ERK biosensor targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev-NLS
   

   Tags

   6xHIS, Nuclear localization signal (NLS), T7 tag …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. hPU.1ΔN165-pET28b

   Plasmid

   #199796

   Purpose

   Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

   Depositor

   Gregory Poon

   Article

   Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

   Insert

   spi1 (SPI1 Human)
   

   Tags

   None

   Expression

   Bacterial

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV7.1_3xFLAG-TAOK2

   Plasmid

   #172992

   Purpose

   constitutive expression of FLAG-tagged TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-TAOK2 (TAOK2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_TP53BP2-1/1

    Plasmid

    #91503

    Purpose

    Protein expression and purification of human SH3 domain construct TP53BP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TP53BP2-1/1 (TP53BP2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-GSDMD-Myc

    Plasmid

    #201166

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-NPC1(P691S)-FLAG

    Plasmid

    #164973

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P691S

    Promoter

    EF1a

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP - FLAG-APEX2- hs Lamin B1

    Plasmid

    #139442

    Purpose

    APEX2-Lamin B1 expression plasmid

    Depositor

    Yixian Zheng

    Article

    Tran et al bioRxiv 2020.02.05.935635

    Insert

    LMNB1 (LMNB1 Human)
    

    Tags

    3xGGGGS linker (between APEX2 and Lamin B1), APEX…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDuet TDP-43 G294A

    Plasmid

    #27463

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pLKO human ULK1 shRNA 8

    Plasmid

    #27633

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    human ULK1 shRNA 8
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_18xTEAD_luc2

    Plasmid

    #173003

    Purpose

    firefly luciferase reporter vector with 18x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    18xTEAD-luc2
    

    Use

    AAV and Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAG sHRPa-FRB-pre mGRASP

    Plasmid

    #73145

    Purpose

    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    sHRPa-FRB-pre mGRASP
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid

    #165430

    Purpose

    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    FHS-paCaMKII(SD)
    

    Use

    AAV

    Tags

    FHS(Flag, HisX6, Strep) tag

    Expression

    Mammalian

    Mutation

    C450A, L514K, G528A, L531E, N538E: Light-insensit…

    Promoter

    CaMKII0.4

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS416 Gal Q331K YFP

    Plasmid

    #27450

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only