We narrowed to 6,908 results for: lentiviral plasmid

1. pLV-EF1A-CD19.28H.28TM.BB.28.3z

   Plasmid

   #200681

   Purpose

   Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX311-SHOC2-V5

   Plasmid

   #134521

   Purpose

   Lentiviral plasmid expressing SHOC2 with V5 C-term tag

   Depositor

   Andrew Aguirre

   Article

   Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Silent mutations made at wobble base position at …

   Promoter

   EF1alpha

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4

   Plasmid

   #193348

   Purpose

   tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4 (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-V1AUC-DelStem-CD20-Puro

   Plasmid

   #209758

   Purpose

   Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Mutations to disrupt the uORFs and the stem-loop …

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17

   Plasmid

   #193299

   Purpose

   tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Klf4-IRES-Sox2-17 (Sox2, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag

   Plasmid

   #192006

   Purpose

   Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-Isoform2-I142V-Flag (LYSET Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMVTRE3G

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307_CR-PDHA2S291A/S293A

   Plasmid

   #115205

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-SARS2-S-D614 (C-flag)

   Plasmid

   #156420

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

   Plasmid

   #173030

   Purpose

   To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   SARS-CoV-2 Nucleocapsid phosphoprotein
   

   Use

   Lentiviral

   Tags

   3xHA and IRES-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Gag (truncated)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

    Plasmid

    #204146

    Purpose

    Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Inserts

    SARS-CoV-2 BA.1 variant spike
    zsGreen-T2A-PuR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    21 amino acid cytoplasmic tail deletion

    Promoter

    CMV and TRE3G

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-puro /BRAF-V600E

    Plasmid

    #131724

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V600E

    Promoter

    CMV

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-MPro-eGFP-2

    Plasmid

    #184824

    Purpose

    Expresses Mpro-eGFP in mammalian cells

    Depositor

    Wenshe Liu

    Article

    Cao et al ACS Cent Sci. 2022 Feb 23;8(2):192-204.

    Insert

    Mpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alfa

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid

    #156421

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-puro/RAF1-S259A

    Plasmid

    #131727

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S259A

    Promoter

    CMV

    Available since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-puro/RAF1-P261A

    Plasmid

    #131728

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P261A

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid

    #156418

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-puro/RAF1-P207S

    Plasmid

    #131726

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P207S

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid

    #171588

    Purpose

    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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