We narrowed to 14,036 results for: TIM

1. NRBP2 gRNA (BRDN0001147825)

   Plasmid

   #76453

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NRBP2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NRBP2 (NRBP2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. NTRK3 gRNA (BRDN0001145325)

   Plasmid

   #76180

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NTRK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NTRK3 (NTRK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR13

   Plasmid

   #69155

   Purpose

   read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   swsn-1 (swsn-1 Nematode)
   eGFP
   

   Use

   Cre/Lox; Mossci

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, codon-optimzed index 1.…

   Promoter

   rps-27 and swsn-1

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. RGS-6xHis-LIN-29-pcDNA3.1-

   Plasmid

   #52515

   Purpose

   expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Lin-29 b (lin-29 Nematode)
   

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   wt (changed coding sequence in 5' (5th codon…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEA1-Puro

   Plasmid

   #171991

   Purpose

   Delivers all prime editing nickase components in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b(+)-Is-PETase

   Plasmid

   #112202

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
   

   Tags

   6XHIS

   Expression

   Bacterial

   Promoter

   N/A

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SspB-HaloTag-p63DH

   Plasmid

   #176129

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Use

   Lentiviral

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SSpB-iRFP-p63DH

   Plasmid

   #176120

   Purpose

   Heterodimerization with iLID, RhoA activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   RHOGEF p63 (ARHGEF25 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCSF-R(mB)-luc

    Plasmid

    #12422

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Mutation

    AML1 binding site mutation (created a KpnI site i…

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

12. BM00369

    Plasmid

    #155338

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189925

    Purpose

    AAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Use

    AAV

    Mutation

    SauriCas9 D15A

    Promoter

    EFS

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. BM00654

    Plasmid

    #155339

    Purpose

    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

    Plasmid

    #189923

    Purpose

    AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SaABE8e V106W
    

    Use

    AAV

    Mutation

    nSaCas9 KKH, TadA ABE8e V106W

    Promoter

    EFS

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SV2C_STOP

    Plasmid

    #161463

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SV2C (SV2C Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pFLAG-YAP1

    Plasmid

    #66853

    Purpose

    Express FLAG-tagged YAP1 in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

    Insert

    YAP1 (YAP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. miABE

    Plasmid

    #205412

    Purpose

    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    

    Expression

    Mammalian

    Mutation

    D61A+E85R+H369R+S387R+S457R

    Promoter

    hU6, CAG, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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