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Addgene

We narrowed to 8,358 results for: BLI

Showing: 6261 - 6280 of 8358 results
  1. p-dCas9-TET1-Hygro

    Plasmid
    #104404
    Purpose
    transient expression of dCas9-TET1 fusion protein
    Depositor
    Insert
    TET1 (TET1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Insert
    PDB:4NW3 (KMT2A Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    ACACA (ACACA Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. Hs.KRAS4b G13D

    Plasmid
    #83133
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G13D
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. DYRK1A

    Plasmid
    #38913
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    DYRK1A (DYRK1A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50470
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  10. pGFP Cas

    Plasmid
    #50729
    Purpose
    mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Insert
    Cas (Bcar1 Rat)
    Use
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  11. Hs.KRAS4b G12D

    Plasmid
    #83131
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. Hs.KRAS4b

    Plasmid
    #83129
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Expression
    Mutation
    See comments
    Promoter
    polyhedrin
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. Hs.KRAS4b G12S

    Plasmid
    #83144
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12S
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. AICSDP-52: HIST1H2BJ-mEGFP

    Plasmid
    #109121
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human HIST1H2BJ
    Insert
    HIST1H2BJ Homology Arms with linker-mEGFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. Hs.KRAS4a

    Plasmid
    #83166
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 6261 - 6280 of 8358 results