Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,474 results for: BLI

Clear keywords
Showing: 6281 - 6300 of 9474 results

  1. pCAGGS-FLAG-BubR1

    Plasmid
    #200814
    Purpose
    expresses BubR1 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200814-plasmid-map-sequence-id-410103

    Enlarge

  2. pAG426-GAL-EWSR1

    Plasmid
    #181725
    Purpose
    Galactose inducible expression of EWSR1
    Depositor
    Alejandro Chavez
    Insert
    EWSR1 (EWSR1 Human)
    Expression
    Yeast
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    181725-plasmid-map-sequence-id-466838

    Enlarge

  3. pWPI-Glis3M412

    Plasmid
    #216854
    Purpose
    This plasmid is for the production of lentiviral vectors expressing an active form of Glis3 together with GFP.
    Depositor
    Patrick Salmon
    Insert
    Glis3 (Glis3 Mouse)
    Use
    Lentiviral
    Mutation
    N-terminal deletion removing repressor domains
    Promoter
    EF1 alpha
    Available Since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    216854-plasmid-map-sequence-id-464607

    Enlarge

  4. pPMS-2397

    Plasmid
    #231044
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to chicken Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    8xHis
    Expression
    Mammalian
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231044-plasmid-map-sequence-id-457178

    Enlarge

  5. pNY-346

    Plasmid
    #231046
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to human Fc
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Available Since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231046-plasmid-map-sequence-id-458329

    Enlarge

  6. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

    Enlarge

  7. pDual_dsCas9_Puro_hs_TP73

    Plasmid
    #214689
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214689-plasmid-map-sequence-id-428791

    Enlarge

  8. pAcGFP-C1-DGKd2

    Plasmid
    #224897
    Purpose
    Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    AcGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    224897-plasmid-map-sequence-id-456312

    Enlarge

  9. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid
    #226590
    Purpose
    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
    Depositor
    D. James Surmeier
    Insert
    PSAML-141F,Y115F-GlyR
    Use
    AAV
    Tags
    P2A-Fusion Red
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    226590-plasmid-map-sequence-id-450965

    Enlarge

  10. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

    Enlarge

  11. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Tags
    6xHis
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available Since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

    Enlarge

  12. N-SF-TAP-DEST-TLK2-D592V

    Plasmid
    #218835
    Purpose
    Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).
    Depositor
    Travis Stracker
    Insert
    TLK2 Q592V (kinase dead) (TLK2 Human)
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    Q592V
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218835-plasmid-map-sequence-id-436652

    Enlarge

  13. 8TBX

    Plasmid
    #218228
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDX1 (DDX1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Promoter
    Polyhedrin
    Available Since
    April 17, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218228-plasmid-map-sequence-id-430075

    Enlarge

  14. pET50_G495R∆2CARD

    Plasmid
    #216805
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Expression
    Bacterial
    Mutation
    G495R/∆1-287
    Available Since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216805-plasmid-map-sequence-id-428836

    Enlarge

  15. 8GCY

    Plasmid
    #214440
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBLB:PBC020-G02:C248501 (CBLB Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214440-plasmid-map-sequence-id-424893

    Enlarge

  16. 8DYS

    Plasmid
    #214439
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EIF2A:PBC022-C06:C245159 (EIF2A Human)
    Use
    Baculovirus
    Tags
    6xHis-TEV
    Promoter
    Polyhedrin
    Available Since
    Feb. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214439-plasmid-map-sequence-id-424355

    Enlarge

  17. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

    Enlarge

  18. pGB-05-06-GST-ATG14

    Plasmid
    #187987
    Purpose
    Plasmid for the protein expression of GST-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1321
    Depositor
    Sascha Martens
    Insert
    ATG14 (ATG14 Human)
    Tags
    GST
    Expression
    Bacterial and Insect
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187987-plasmid-map-sequence-id-374440

    Enlarge

  19. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available Since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

    Enlarge

  20. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183026
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Bacterial and Mammalian
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183026-plasmid-map-sequence-id-360495

    Enlarge

Showing: 6281 - 6300 of 9474 results