We narrowed to 8,358 results for: BLI

1. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50464

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. psd44-G12QL

   Plasmid

   #46825

   Purpose

   expression of mutant G protein alpha 12 in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q231L (the mutation reduces GTPase activity resul…

   Promoter

   PGK

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Flex-Axon-GCaMP7b

   Plasmid

   #135419

   Purpose

   Can be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.

   Depositor

   Rylan Larsen

   Insert

   axon-jGCaMP7b
   

   Use

   AAV

   Tags

   6xHis and GAP43 palmitoylation domain

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50471

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   GFAP

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAMK2G

   Plasmid

   #38894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2G (CAMK2G Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

   Plasmid

   #50467

   Purpose

   Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV2 and AAV8

   Insert

   hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   CaMKIIa

   Available since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426-GAL-Alpha-synuclein-E46K

   Plasmid

   #181723

   Purpose

   Galactose inducible expression of Alpha-synuclein-E46K

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   E46K

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4a G12D

    Plasmid

    #83168

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDONR1K18ms

    Plasmid

    #72644

    Purpose

    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

    Depositor

    Brian Kvitko

    Article

    Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

    Type

    Empty backbone

    Use

    Sucrose counter-selection allelic exchange vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAGGS-N2c(G)

    Plasmid

    #73481

    Purpose

    N2c Glycoprotein expression vector

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    N2c(G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hs.NRAS G12V

    Plasmid

    #83174

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SARS-CoV-2 Spike-d18

    Plasmid

    #164583

    Purpose

    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

    Depositor

    Jason Sheltzer

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1256-1273

    Promoter

    EF1a

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ALDH18A1

    Plasmid

    #38947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. DDX3X in pENTR

    Plasmid

    #216572

    Purpose

    gateway cloning donor vector containing DDX3X

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Expression

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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