We narrowed to 15,724 results for: LEA

1. pBABE-Flag-Cdk9-IRES-eGFP

   Plasmid

   #28096

   Depositor

   Andrew Rice

   Article

   Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

   Insert

   Cdk9 (CDK9 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 15, 2011

   Availability

   Academic Institutions and Nonprofits only

2. PD-1-miSFIT-4x

   Plasmid

   #124678

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Expression

   Mammalian

   Promoter

   minimal CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

   Plasmid

   #127354

   Purpose

   gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, V5, and mVenus

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCdOpt-BMX

   Plasmid

   #203929

   Purpose

   This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

   Depositor

   Scott Briggs

   Article

   Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

   Insert

   BleMX
   

   Expression

   Yeast

   Mutation

   BleMX was codon optimized for Candida albicans or…

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRCH-YE1-BE4max

   Plasmid

   #198549

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-YE1-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWXPGR-pTF-Wnt3a

   Plasmid

   #114287

   Purpose

   TET-inducible expression of Wnt3a

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   Wnt3a (Wnt3a Mouse)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. plentiCMV10x-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196513

   Purpose

   Express single-chain pMHC with sequencing tag for 10x genomics

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C, 10xgenomics compatible tag

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-hARdeltaNH2-6

   Plasmid

   #89104

   Purpose

   mammalian expression of human androgen receptor amino acids 507-919

   Depositor

   Elizabeth Wilson

   Article

   Simental et al J Biol Chem. 1991 Jan 5;266(1):510-8.

   Insert

   hAR DNA binding domain+ligand binding domain (AR Human)
   

   Expression

   Mammalian

   Mutation

   NH2 terminal deletion, contains amino acids 507-…

   Promoter

   CMV

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5_T7-GAP43-mScarlet3

   Plasmid

   #225932

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   GAP43-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ shIFNAR

    Plasmid

    #127699

    Purpose

    Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shIFNAR1 Mmu (Ifnar1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV SNAP MDC1

    Plasmid

    #227375

    Purpose

    SNAP–MDC1 expression

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST PTBP1 isoform 4

    Plasmid

    #108594

    Purpose

    Recombinant GST tagged protein production

    Depositor

    Chris Smith

    Article

    Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

    Insert

    PTBP1 isoform 4 (PTBP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    isoform 4 (isoform a)

    Promoter

    tac

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-ABE7.9

    Plasmid

    #102918

    Purpose

    expresses ABE7.9 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.9
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-NurRE3

    Plasmid

    #208253

    Purpose

    pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

    Depositor

    Douglas Kojetin

    Article

    Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

    Insert

    3X Nur response element LUC
    

    Tags

    No

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-vCre

    Plasmid

    #55638

    Purpose

    EF1a-driven expression of VCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV8

    Insert

    vCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cre-p2a-tdTomato

    Plasmid

    #176837

    Purpose

    Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Cre-P2A-tdTomato
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE-Flag-Cdk9-D167N-IRES-eGFP

    Plasmid

    #28098

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains D167N mutation in Cdk9

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CaMKII-GRAB_g5-HT3.0

    Plasmid

    #208723

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    CaMKII

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY002 (pFnCpf1_min)

    Plasmid

    #69975

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes nd artificial promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-Flag-Cdk9-T186A-IRES-eGFP

    Plasmid

    #28097

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Available Since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only