We narrowed to 10,337 results for: plasmids 101
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Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hChATp-tdTomato
Plasmid#197564PurposeExpresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragmentDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhuman ChAT promoterAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc17a7
Plasmid#124846PurposeMutagenesis of Slc17a7DepositorInsertSlc17a7 (Slc17a7 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(TIAM1)
Plasmid#85156Purposeacute activation of Rac through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV/L7-6-GFP-WPRE
Plasmid#126462PurposeAn AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.DepositorInsertL7-6, Purkinje cell specific promoter
UseAAVTagsExpressionMammalianMutationPromoterAvailable SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)3
Plasmid#127148PurposeMammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-135 mRFP
Plasmid#206927PurposeThe micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.DepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterMP-135Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV CaMKIIa iChloC 2A tDimer
Plasmid#66709PurposeChloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent proteinDepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVTagsExpressionMammalianMutationE83Q,E90R,E101S,D156N,T159CPromoterCaMKIIaAvailable SinceOct. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1
Plasmid#205447Purposehuman AAVS1-targeting plasmid for expression of mouse CR1DepositorInsertmCR1
UseTagsExpressionMammalianMutationPromoterEF1aAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBRed-d2-hygro
Plasmid#139196Purposenon-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puroDepositorInsertCBRed
UseLuciferase and RetroviralTagsd2 PEST domain and nuclear localization signal (N…ExpressionMammalianMutationPromoterAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40
Plasmid#216533PurposeAn axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switchDepositorInsertaxon-jGCaMP8m
UseAAV and Cre/LoxTagsGAP43ExpressionMammalianMutationPromoterhSynapsin1Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-TVAmCherry-2A-oG
Plasmid#102985PurposeAAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombinationDepositorInsertsTVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination
UseAAV; Adeno associated viral vectorTagsExpressionMutationPromoterAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Blasticidin-CAG-Flpe-ERT2
Plasmid#68461PurposeAAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection geneDepositorInsertmammalian-codon-optimized Flpe recombinase fused with ERT2
UseSynthetic BiologyTagsERT2ExpressionMammalianMutationPromoterCAGAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-jGCaMP7f-WPRE
Plasmid#203848PurposeExpression of GCaMP7f in CaMKII expressing cellsDepositorInsertjGCaMP7f
UseAAVTagsExpressionMutationPromoterAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoECDS
Plasmid#41848DepositorAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Erg
Plasmid#66977PurposeExpression plasmid for mouse Erg; adds Flag tag to the N-terminus of proteinDepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFNC-3
Plasmid#233296PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
UseTagsExpressionBacterialMutationPromoterPEM7 and unknownAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Rfx4
Plasmid#61544PurposeExpresses Rfx4 in mammalian cellsDepositorAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only