We narrowed to 6,525 results for: sas

1. pBMN_HA-TBK1 (kinase dead - D135N)

   Plasmid

   #199206

   Purpose

   Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D135N

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1A-mSTING

   Plasmid

   #229739

   Purpose

   Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   STING (Sting1 Mouse)
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

   Plasmid

   #86851

   Purpose

   mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP6 (ATP6 Human)
   

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon corrected

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LiOn-CAG∞IRFP

   Plasmid

   #154017

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   IRFP670
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT2K-CAGGS-T2TP

   Plasmid

   #114725

   Purpose

   expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   Tol2 transposase
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-EF1A-Puro-mCherry-RAB7

   Plasmid

   #221555

   Purpose

   Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Duet1-His-TEV-mCherry-OPTN WT

   Plasmid

   #190191

   Purpose

   Plasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Tags

   6xHis, TEV, and mCherry

   Expression

   Bacterial

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mcherry p62 delta UBA silent

   Plasmid

   #187984

   Purpose

   mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lacking UBA domain (aa389-434) but it has the sil…

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mcherry-p62 delta PB1 silent

   Plasmid

   #187985

   Purpose

   human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

   Depositor

   Sascha Martens

   Article

   Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

   Insert

   p62 (NUP62 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lacking PB1 domain (aa1-102) with a silent mutati…

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid

    #216763

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ePB-PURO-FLAG-METTL3

    Plasmid

    #160252

    Purpose

    For stable integration of FLAG-METTL3 in human cells with trasposase

    Depositor

    Alessandro Fatica

    Article

    Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2/GD-IRES-GFP-CTNNB1

    Plasmid

    #192868

    Purpose

    Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

    Depositor

    David Largaespada

    Article

    Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

    Inserts

    luc+
    EGFP
    CTNNB1
    

    Expression

    Mammalian

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0747 (pDonor_L-MmeI)

    Plasmid

    #130647

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (L*)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Mutation

    TGTTGATG --> TGTTGGAG

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

    Plasmid

    #190237

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0746 (pDonor_R-MmeI)

    Plasmid

    #130646

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (R*)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Mutation

    TGTTGATA --> TGTTGGAA

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T1_GST-thrombin-TEX264(28-313aa)

    Plasmid

    #227714

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    TEX264(28-313aa) (TEX264 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T1_GST-thrombin-FAM134C(250-466aa)

    Plasmid

    #227715

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    FAM134C(250-466aa) (RETREG3 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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