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pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Publication

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Tags

pLacZ-EYFP

Expression

Bacterial

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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ZNF668-Flag

Plasmid

#42636

Depositor

Shiaw-Yih Lin

Publication

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZNF668 (ZNF668 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
pDN-G1TGt

Plasmid

#44508

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Publication

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcsII-pb2-flag

Plasmid

#86766

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 2 (PSMB2 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDisplay-DNER

Plasmid

#51053

Purpose

expression of HA-DNER in mammalian cells

Depositor

Mineko Kengaku

Publication

Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

Insert

DNER (Dner Mouse)

Tags

HA and signal peptide

Expression

Mammalian

Mutation

without endogenous signal peptide (1-221bp)

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLVX-Puro-mGSDME F2A

Plasmid

#154878

Purpose

Expresses mGSDME F2A in mammalian cells

Depositor

Judy Lieberman

Publication

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Mutation

changes Phenylalanine 2 to Alanine

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-Pa01-bpNLS-3'UTR-polyA (CJT484)

Plasmid

#248218

Purpose

Pa01-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-Pa01-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-PE7-3'UTR-polyA (CJT612)

Plasmid

#248222

Purpose

PE7 IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-PE7-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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T7-5'UTR-SuperDn29-bpNLS-3'UTR-polyA (CJT611)

Plasmid

#248220

Purpose

bpNLS-SuperDn29 IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-SuperDn29-bpNLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Super Dn29(M6I, E70G, A224P, G227V, I233K, K248R,…

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Dn29 attD pDonor (CJT454)

Plasmid

#248228

Purpose

Dn29 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Dn29 attD

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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Pa01 attP60 pDonor (CJT460)

Plasmid

#248225

Purpose

Pa01 attP pDonor with Nt.BbvcI site for cssDNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

Pa01 attP

Use

CRISPR; In vitro transcription

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

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LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR (DRR724)

Plasmid

#248231

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attB target sites

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attB)-spacer-BceINT(attB)-spacer-Pa01(attB)-spacer-SacINT(attB)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604)

Plasmid

#248210

Purpose

SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SV40NLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

SV40NLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-Letm1shRNA-hPGK-Puro

Plasmid

#215362

Purpose

Expresses a rat Letm1 specific shRNA

Depositor

Jaime de Juan-Sanz

Publication

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Letm1 shRNA Target sequence (Letm1 Rat)

Use

Lentiviral

Available since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pGGG L2 TaStb15

Plasmid

#226630

Purpose

Wheat transformation vector used to express wheat Septoria tritici blotch (Stb) resistance gene 15

Depositor

Brande Wulff

Publication

Hafeez et al Nat Plants. 2025 Mar;11(3):410-420. doi:

Insert

Triticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene

Use

Synthetic Biology

Expression

Plant

Promoter

Native TaStb15

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AURKA-Aquamarine

Plasmid

#228564

Purpose

Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

Depositor

Giulia Bertolin

Publication

Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

Insert

AURKA (AURKA, AURKA Human)

Tags

Aquamarine

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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