We narrowed to 13,163 results for: CAR

1. pET28a-P5CR

   Plasmid

   #110295

   Purpose

   Expresses pyrroline-5-carboxylate reductase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Pyrroline-5-carboxylate Reductase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJP72

   Plasmid

   #176479

   Purpose

   Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Inserts

   actin > GenR
   EF1 > mScarlet
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPC027.306

   Plasmid

   #171149

   Purpose

   Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAN1735

   Plasmid

   #127204

   Purpose

   Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS

   Depositor

   Hana El-Samad

   Article

   Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

   Insert

   pGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
   

   Use

   Synthetic Biology

   Available Since

   June 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. M48 GST-TEV-Chibby-N

   Plasmid

   #17196

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   Chibby (CBY1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-63

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. 1kb Zfp521 promoter reporter

   Plasmid

   #104188

   Purpose

   luciferase reporter to assay Zfp521 promoter activation

   Depositor

   Kathryn Hentges

   Article

   Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

   Insert

   Zfp521 promoter - 1 Kb (Zfp521 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AB.pCCL.sin.cPPT.U6.miR-16-Decoy.hPGK.GFP.WPRE

   Plasmid

   #46593

   Depositor

   Brian Brown

   Article

   Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

   Insert

   miR-16 Decoy (Mir16-1 Human, Mouse)
   

   Use

   Lentiviral

   Promoter

   hU6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pUASP dsRed Rab11 DN

   Plasmid

   #44831

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab11 (Rab11 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   S25N (dominant negative)

   Available Since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pPN021

    Plasmid

    #91667

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM1162

    Plasmid

    #112535

    Purpose

    pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Expression

    Bacterial

    Promoter

    J23107 and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid

    #119867

    Purpose

    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-TEVcs-GFP-ATG101

    Plasmid

    #171415

    Purpose

    For the purifcation of GFP-ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG101 (ATG101 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUASP dsRed Rab7 CA

    Plasmid

    #44825

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pILGFP86

    Plasmid

    #83543

    Purpose

    Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL4A promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    RPL4A

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-OspCas12c-noncoding

    Plasmid

    #120878

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-OspCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-GFP-ATG14

    Plasmid

    #171426

    Purpose

    For the purifcation of GFP- PI3KC3 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ATG14 (ATG14 Human)
    

    Expression

    Mammalian

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. M49 GST-TEV-Chibby-C

    Plasmid

    #17197

    Depositor

    Randall Moon

    Article

    Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

    Insert

    Chibby (CBY1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 64-126

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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