We narrowed to 14,036 results for: TIM

1. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACE2(WT)-Fc(IgA1)

   Plasmid

   #145154

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_Xph20-mNeonGreen-CCR5TC

   Plasmid

   #135534

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG_Xph15-mNeonGreen-CCR5TC

   Plasmid

   #135533

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15-ZmPYK

   Plasmid

   #220230

   Purpose

   Inducible expression of Zymomonas mobilis pyruvate kinase

   Depositor

   Aron Fenton, Liskin Swint-Kruse

   Article

   Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

   Insert

   Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET.GltI253-cpGFP.L1LV/L2NP

   Plasmid

   #41733

   Purpose

   a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Insert

   GltI based Glutamate Biosensor
   

   Use

   Glutamate biosensor

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

   Promoter

   T7

   Available Since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIG-FGFRdn

   Plasmid

   #80431

   Purpose

   Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

   Depositor

   Aixa Morales

   Article

   Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

   Insert

   Fibroblast growth factor receptor 1 (FGFR1 Chicken)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa 1–425

   Promoter

   beta-actin promoter

   Available Since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pLL3.7-CAG-hMGT-Ef1a-puro

   Plasmid

   #172395

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLL3.7-UBCp-hMGT-Ef1a-puro

   Plasmid

   #172396

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDyn1

    Plasmid

    #64067

    Purpose

    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    DYNC1H1 (DYNC1H1 Human)
    

    Use

    Cre/Lox

    Tags

    His-ZZ-TCS-SNAPf

    Expression

    Insect

    Mutation

    Codon optimized for Sf9

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_Xph15-SNAPf-CCR5TC

    Plasmid

    #135536

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Tags

    SNAPf

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid

    #139993

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid

    #123181

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LeGO-iBSD-5LO-WT

    Plasmid

    #182741

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-SLC25A30_STOP

    Plasmid

    #161218

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A30 (SLC25A30 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDONR221-SLC26A6_STOP

    Plasmid

    #161222

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC26A6 (SLC26A6 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-sACE2(WT)

    Plasmid

    #145147

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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