We narrowed to 59,355 results for: cloning

1. pRS423-ProSgH

   Plasmid

   #163974

   Purpose

   Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-HIPK3-V5-miniTurbo_IDG-K

   Plasmid

   #158191

   Purpose

   Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   HIPK3-V5-miniTurbo (HIPK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFH50

   Plasmid

   #128212

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAK007

   Plasmid

   #128220

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH72

   Plasmid

   #128221

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFH73

   Plasmid

   #128222

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU6-2 promoter module (pFH36)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFH74

   Plasmid

   #128223

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)

   Plasmid

   #123542

   Purpose

   MoChlo kit

   Depositor

   Scott Lenaghan, C. Neal Stewart

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   psbA-rbcL chimeric 5'UTR (R delta 5'SL)
   

   Use

   Synthetic Biology

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. kank1b_pJet1.2

   Plasmid

   #121979

   Purpose

   for generating kank1b riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank1b (si:dkey-226m8.9 Zebrafish)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mdm1_pJet1.2

    Plasmid

    #121977

    Purpose

    for generating mdm1 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

    Insert

    mdm1 (mdm1 Zebrafish)
    

    Use

    Cloning vector

    Promoter

    T7

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-R4R3-mRuby-N

    Plasmid

    #113752

    Purpose

    Gateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mRuby2

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-R4R3-2xmEGFP-N

    Plasmid

    #113747

    Purpose

    Gateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    2xmEGFP

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-R4R3-3xmEGFP-N

    Plasmid

    #113748

    Purpose

    Gateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmEGFP

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-R4R3-2xmEGFP-C

    Plasmid

    #113750

    Purpose

    Gateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP-mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    2xmEGFP

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-R4R3-2xmRuby-N

    Plasmid

    #113753

    Purpose

    Gateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    2xmRuby2

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-R4R3-2xmRuby-C

    Plasmid

    #113756

    Purpose

    Gateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    2xmRuby2

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK26

    Plasmid

    #105429

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Bs3
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM16841

    Plasmid

    #105416

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    STOP codon and nos terminator
    

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOG267

    Plasmid

    #105339

    Purpose

    Synthetic biology; gateway destination vector assembly

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUPD-MTS2 (12)

    Plasmid

    #101698

    Purpose

    MTS2 in domestication vector

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Insert

    CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG
    

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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