We narrowed to 14,023 results for: egf

1. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

   Plasmid

   #129708

   Purpose

   Express cre-dependent KASH-EGFP and scramble shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217769

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Sleeping beauty transposase-compatible genome ins…

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CA3 domain aa 317-400

   Promoter

   EF-1alpha promoter

   Available Since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC-attB-burs-mCD8-EGFP-T2A-Gal4

   Plasmid

   #39463

   Depositor

   Benjamin White

   Article

   Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

   Insert

   burs-mCD8-EGFP-T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bursalpha

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

   Plasmid

   #162084

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

   Plasmid

   #162091

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Puro DEST (w118-1) eGFP

   Plasmid

   #107505

   Purpose

   Expresses eGFP, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

   Plasmid

   #87828

   Purpose

   High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-Gluc-RMA-IRES-EGFP

    Plasmid

    #189629

    Purpose

    Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and hSyn

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICOZ-SB100X[EF1α-CD19 4-1BB-eGFP]

    Plasmid

    #218343

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. XLone-eGFP NLS (no drug resistant gene)

    Plasmid

    #140032

    Purpose

    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

    Plasmid

    #183352

    Purpose

    Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    TVA, EGFP and tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0303 SFFV-mCherry-SG(Bdnf.s3)SG-EGFP (FLP-IN)

    Plasmid

    #191180

    Purpose

    Negative control sensor in human cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s3:EGFP (negative control)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-CMV-Rheb(D60K)-IRES-eGFP-W

    Plasmid

    #32522

    Depositor

    Mustafa Sahin

    Insert

    dominant negative Rheb (D60K) (Rheb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D60K to make dominant negative

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

    Plasmid

    #205549

    Purpose

    Express EPN24-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid

    #193051

    Purpose

    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    ALA7-TMD
    

    Use

    Tags

    Ig k-chain leader sequence, MBP, and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP

    Plasmid

    #209194

    Purpose

    Cre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcript

    Depositor

    Larry Zweifel

    Article

    Gamal El-Din et al Proc Natl Acad Sci U S A. 2021 Nov 9;118

    Insert

    Kcnq3 (KCNQ3 Human)
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R230 to C230

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid

    #140026

    Purpose

    Tunable and temporal expression control of NFIL3 and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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