We narrowed to 88,640 results for: Mal

1. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDMS151A_Tet-On-3G

   Plasmid

   #191577

   Purpose

   ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

   Depositor

   Joshua Leonard

   Article

   Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

   Insert

   Tet-On(R) 3G
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWN_U6-B2M-miniGag

   Plasmid

   #228957

   Purpose

   miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag
   

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-CaM-uTEVp

   Plasmid

   #163032

   Purpose

   FLiCRE protease for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   GFP-CaM-uTEVp
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tad-CBE(CCG3)

   Plasmid

   #235074

   Purpose

   Expresses Tad-CBE(CCG3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCG3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3336 pcDNA3 Bcl-2

   Plasmid

   #8768

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-HypaCas9-AAVS1_sgRNA

   Plasmid

   #183891

   Purpose

   pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRPML1-YFP

   Plasmid

   #18826

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML1 (MCOLN1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. mScarlet-I-UtrCH

   Plasmid

   #98823

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   UtrCH
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hPGK and miniCMV

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Ras-LOCKR-S R89L (negative control)

    Plasmid

    #216497

    Purpose

    FRET-based reporter for Ras activity, untargeted, negative control

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S R89L negative control
    

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROP_C9_Puro_ERBB2

    Plasmid

    #183287

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    ERBB2
    

    Use

    Lentiviral

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ABE8r-V106W

    Plasmid

    #208295

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry- eMagBF - 5ptaseOCRL

    Plasmid

    #162254

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    mCherry- eMagBF - 5ptaseOCRL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Neo(+) hSUMF1

    Plasmid

    #24386

    Depositor

    Carolyn Bertozzi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    hSUMF1 (SUMF1 Human)
    

    Expression

    Mammalian

    Available Since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. EKAREN5-gl

    Plasmid

    #225958

    Purpose

    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

    Depositor

    Toru Hiratsuka

    Article

    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

    Insert

    EKAREN5-gl
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Promoter

    EF-1-alpha promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. xCas9 3.7

    Plasmid

    #108379

    Purpose

    Mammalian expression vector for xCas9 3.7

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.7
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-GFP

    Plasmid

    #137763

    Purpose

    Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    GFP
    

    Tags

    HA and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG BacMam C term 3C eGFP StrepII

    Plasmid

    #160686

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    3C eGFP StrepII

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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