We narrowed to 15,723 results for: LEA

1. pNS20-SpCas9-SNAP

   Plasmid

   #113717

   Purpose

   Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSP2135

   Plasmid

   #72248

   Purpose

   Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, and D1135E

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1_T7-mScarlet3-Hras

   Plasmid

   #225928

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-Ras membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-HRas
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox

   Plasmid

   #196889

   Purpose

   Dre-activated Cre-inactivated expression of lyn-mScarlet reporter.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   lyn-mScarlet
   

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRMC2-tat:msfGFP

   Plasmid

   #194915

   Purpose

   Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Expression

   Bacterial

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-FLEX-ArgiNLS-AausFP1

   Plasmid

   #220602

   Purpose

   Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   ArgiNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

   Plasmid

   #176281

   Purpose

   Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBacMUT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   E191K

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin

   Plasmid

   #134869

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome b5 type A (CYB5A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-98

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-pSFFV-TAF15N-mCherry-sspB

   Plasmid

   #122436

   Purpose

   Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-208

   Promoter

   SFFV

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUG-T2A-Pten

    Plasmid

    #140738

    Purpose

    Lentiviral vector to overexpress human PTEN and GFP

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-PTA-PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Promoter

    hUbiC

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-sCre

    Plasmid

    #55635

    Purpose

    Expresses sCre in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-eSpCas9-plus-NLS-6xHis

    Plasmid

    #207380

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, R1060A, amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-BG505.SOSIP-TM

    Plasmid

    #111844

    Purpose

    Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Tags

    CD5 leader sequence and TM helix from MHC class I

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 2_T7-mScarlet3-KRas

    Plasmid

    #225929

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS2155

    Plasmid

    #141065

    Purpose

    AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

    Insert

    SYN1-EmGFP-WPRE
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    April 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PD-L1-miSFIT-Ce67

    Plasmid

    #124686

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-L1 (CD274 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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