We narrowed to 15,599 results for: LEA

1. pAAV-Ef1a-DIO-VGAT-WPRE-pA

   Plasmid

   #39320

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV; R

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX_317-C7orf26v1

   Plasmid

   #178537

   Purpose

   Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v1 (INTS15 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI-Flag-NEDD4L∆WW1,2,4

   Plasmid

   #171137

   Purpose

   expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 221 to alanine, tryptophan 453…

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICSL11055

   Plasmid

   #68252

   Purpose

   Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpIMPDH

   Plasmid

   #206980

   Purpose

   Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

   Depositor

   Vern Schramm

   Article

   Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

   Insert

   SpIMPDH (guaB Streptococcus pyogenes)
   

   Use

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICE-HA-CtIP-siR-WT

   Plasmid

   #82030

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutations making the cDNA resistant to a siRNA

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

   Plasmid

   #129394

   Purpose

   Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   soma targeted ChRger2
   

   Use

   AAV and Cre/Lox

   Tags

   Kv2.1-TS-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-KLF3

   Plasmid

   #49100

   Purpose

   Expresses KLF3 in mammalian cells

   Depositor

   Merlin Crossley

   Article

   Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

   Insert

   Krüppel-Like Factor 3(KLF3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

9. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

   Plasmid

   #176839

   Purpose

   To recruit SAM complex to human CYP3A4 proximal enhancer.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   Human CYP3A4 proximal enhancer sgRNA for activation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Tags

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3E_BAG3P209L-eGFP

    Plasmid

    #98181

    Purpose

    Codes for full length, GFP tagged mutant Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3P209L-eGFP (BAG3 Human)
    

    Use

    Gateway vector

    Tags

    GFP

    Expression

    Mutation

    P209L

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. MDH1-miR-221-PGK-GFP-Blast

    Plasmid

    #31519

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. shFGF2#2

    Plasmid

    #157668

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Use

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEmerald-Actin-C-18

    Plasmid

    #53978

    Purpose

    Localization: Actin, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

    Plasmid

    #169126

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NAWLVH106-AAAAAA

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-TetO-Tfap2c

    Plasmid

    #128826

    Purpose

    dox-dependent expression of mouse Tfap2c

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Tfap2c (Tfap2c Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge