We narrowed to 11,691 results for: NSI

1. C1-FKBP(F36V):mCherry-Akt2(K14A)

   Plasmid

   #72906

   Purpose

   Recruitable Akt2(K14A) mutant

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   RAC-beta serine/threonine-protein kinase (AKT2 Human)
   

   Tags

   FKBP(F36V), mCherry

   Expression

   Mammalian

   Mutation

   K14A mutation

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-H2B-6

   Plasmid

   #54111

   Purpose

   Localization: Nucleus/Histones, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. CytERM-mAvicFP1

   Plasmid

   #129510

   Purpose

   Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   CytERM-mAvicFP1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAdEasy-hALDOA

   Plasmid

   #210812

   Purpose

   To package adenovirus expressing wild-type aldolaseA

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Adenoviral

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC_N_ARP3B

   Plasmid

   #111680

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   ARP3B (ACTR3B Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAav-MCS-PQS2-3xHA

   Plasmid

   #84917

   Purpose

   rAAV-based template for genome engineering of protein C-termini containing PQS2 and 3xHA tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Insert

   LOX-PGK-NEO-LOX
   

   Use

   AAV

   Tags

   PQS2 3xHA

   Promoter

   mPGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-CCHD (p130Cas)

   Plasmid

   #145133

   Purpose

   Expresses CCH domain from p130Cas with an mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Jacquemet et al Curr Biol. 2019 Jan 21;29(2):202-216.e7.

   Insert

   p130Cas CCH domain (BCAR1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGLS3-5xHRE-PHLDA3(wt)

   Plasmid

   #72557

   Purpose

   Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   PHLDA3 (PHLDA3 Human)
   

   Tags

   5XHRE

   Expression

   Mammalian

   Promoter

   E1b minimal

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_NOXO1-1/2

    Plasmid

    #91499

    Purpose

    Protein expression and purification of human SH3 domain construct NOXO1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NOXO1-1/2 (NOXO1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PAmCherry1-N1-Lyn11

    Plasmid

    #202683

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    LYN(1-11):PAmCherry1 (LYN Human)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LAP2-beta-actin in modified pEGFP

    Plasmid

    #34839

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LAP2-beta-Actin (ACTB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pHH0103_SHANK3-1/1

    Plasmid

    #91421

    Purpose

    Protein expression and purification of human SH3 domain construct SHANK3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SHANK3-1/1 (SHANK3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pRS416 Gal TDP43 M337V

    Plasmid

    #27460

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pRS416 Gal TDP43 Q331K

    Plasmid

    #27461

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

17. tdp43-EGFP construct10

    Plasmid

    #28203

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-105 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_TNK1-1/1-SV1/3

    Plasmid

    #91548

    Purpose

    Protein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TNK1-1/1-SV1/3 (TNK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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