We narrowed to 52,753 results for: plasmid
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pRSET.HaloTag.v7.CoOp.Cys-less
Plasmid#195487PurposeBacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.DepositorInsertHaloTag.v7
UseTags6xHISExpressionBacterialMutationC61T, C262VPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH
Plasmid#100050PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorHas ServiceAAV9InserthChR2(E123T/H134R)
UseAAVTagsEYFPExpressionMammalianMutationhumanized, E123T/H134RPromoterCaMKIIaAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RB-PE2
Plasmid#173903PurposeSB-transposon with inducible expression of SpCas9 PE2DepositorInsertPE2
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pZBTB5-donor
Plasmid#112303PurposeCRISPR donor plasmid to tag human transcription factor ZBTB5 with GFPDepositorInsertZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W
Plasmid#32521DepositorInsertDominant negative Rheb (D60I) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationD60I to make dominant negativePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH
Plasmid#100049PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activationDepositorHas ServiceAAV9InserthChR2(E123T/H134R)
UseAAVTagsEYFPExpressionMammalianMutationhumanized, E123T/H134RPromoterhSynapAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxb1a
Plasmid#27519DepositorInsertHoxb1a (hoxb1a Zebrafish)
UseRiboprobe synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa-C1V1::FusionRed::Kv2.1
Plasmid#102771Purpose"Activate neurons with 2p stimulation"DepositorInsertC1V1::FusionRed::Kv2.1
UseAAVTagsFusionRed and Kv2.1 traficking domainExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBS ZfHoxa2b
Plasmid#27514DepositorInsertHoxa2b (hoxa2b Zebrafish)
UseRiboprobe synthesisTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
psicheck_NDUFA6_3UTR
Plasmid#65886PurposeLuciferase assay vector, assessing regulation of NDUFA6 3' UTRDepositorInsertNDUFA6 (NDUFA6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS ZfHoxc6a
Plasmid#27541DepositorInsertHoxc6a (hoxc6a Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGloSensor -30F DEVD Caspase Vector
Plasmid#236880PurposeExpress -30F DEVD for Caspase 3/7 assayDepositorHas ServiceDNAInsert-30F DEVD
UseLuciferaseTagsExpressionMutationNonePromoterCMVAvailable sinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHnS 2comp Donor;smFP-V5 KO;Template ORF2
Plasmid#240306PurposeDonor:smFP-V5 KO:TemplateDepositorInsertTemplate gRNA
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS 2comp Donor;smFP-V5 KO;Template ORF1
Plasmid#240305PurposeDonor:smFP-V5 KO:TemplateDepositorInsertTemplate gRNA
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS KI;Actb Donor;3xV5 KO;Template
Plasmid#240291PurposeKI:Actb Donor:3xV5 KO:TemplateDepositorInsertKI gRNA for Actb
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS KI;Sptbn4 Donor;3xV5 KO;Template
Plasmid#240295PurposeKI:Sptbn4 Donor:3xV5 KO:TemplateDepositorInsertKI gRNA for Sptbn4
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS KI;Gria1 Donor;3xV5 KO;Template
Plasmid#240293PurposeKI:Gria1 Donor:3xV5 KO:TemplateDepositorInsertKI gRNA for Gria1
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS KI;Pdha1 Donor;3xV5 KO;Template
Plasmid#240300PurposeKI:Pdha1 Donor:3xV5 KO:TemplateDepositorInsertKI gRNA for Pdha1
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHnS KI;Lmnb1 Donor;3xV5 KO;Template
Plasmid#240297PurposeKI:Lmnb1 Donor:3xV5 KO:TemplateDepositorInsertKI gRNA for Lmnnb1
UseAAVTagsExpressionMutationNAPromoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2 GATD1 Alu_Mut2
Plasmid#205471PurposeLuciferase reporter for Alu Domain ActivityDepositorInsertMutant 2 GATD1 Alu Domain
UseLuciferase and RNAiTagsExpressionMutationMutated the arms of the alu domain by adding ACAC…PromoterT7Available sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-KAE1
Plasmid#232891PurposePlasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.DepositorInsertKAE1 gRNA (KAE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SAM50
Plasmid#232895PurposePlasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.DepositorInsertSAM50 gRNA (SAM50 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS1_NSP13_BV_NBIO-TEV_CTHROM-his
Plasmid#234382PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_SARS
UseBaculovirus expresssionTagsLVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ARB1
Plasmid#232888PurposePlasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.DepositorInsertARB1 gRNA (ARB1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_DDX25
Plasmid#232359PurposeEnhancer near DDX25, chr11:125935511-125936012 cloned into pGL3DepositorInsertDDX25 (DDX25 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_MSR1_bothneg0scramble
Plasmid#232383PurposeMSR1 enhancer, both buffering Coordinators scrambledDepositorInsertMSR1 (MSR1 Human)
UseLuciferaseTagsExpressionMutationMSR1 enhancer, both buffering Coordinators scramb…PromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_FAM153CP_SOX9rep
Plasmid#232400PurposeEnhancer near FAM153CP, buffering element replaced with SOX9 siteDepositorInsertFAM153CP (FAM153CP Human)
UseLuciferaseTagsExpressionMutationEnhancer near FAM153CP, buffering element replace…PromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-RER2
Plasmid#232884PurposePlasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.DepositorInsertRER2 gRNA (RER2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-3
Plasmid#232894PurposePlasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.DepositorInsertLEU2 gRNA (RER2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-HygMx4-URA3-3
Plasmid#232886PurposePlasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.DepositorInsertURA3 gRNA (URA3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-HygMx4-URA3-2
Plasmid#232885PurposePlasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.DepositorInsertURA3 gRNA (URA3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZP05
Plasmid#232319PurposepZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatumDepositorInsertOri-repA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-AVO1
Plasmid#232889PurposePlasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.DepositorInsertAVO1 gRNA (AVO1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-YFR054C
Plasmid#232900PurposePlasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.DepositorInsertYFR054C gRNA (YFR054C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ZIM17
Plasmid#232901PurposePlasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.DepositorInsertZIM17 gRNA (ZIM17 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SRP14
Plasmid#232896PurposePlasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.DepositorInsertSRP14 gRNA (SRP14 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-TLG1
Plasmid#232897PurposePlasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.DepositorInsertTLG1 gRNA (TLG1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VHT1
Plasmid#232898PurposePlasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.DepositorInsertVHT1 gRNA (VHT1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ADE13
Plasmid#232887PurposePlasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.DepositorInsertADE13 gRNA (ADE13 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-LAS17
Plasmid#232892PurposePlasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.DepositorInsertLAS17 gRNA (LAS17 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-2
Plasmid#232883PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.DepositorInsertLEU2 gRNA (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS313-rad53-5004
Plasmid#232902PurposePlasmid carrying the rad53-5004 allele with its native promoter and terminator.DepositorInsertRAD53 (RAD53 Budding Yeast)
UseTagsExpressionYeastMutationT600C, A680G, A1094T, C1615T, C2228APromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-INO80
Plasmid#232890PurposePlasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.DepositorInsertINO80 gRNA (INO80 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterGPDAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gutB
Plasmid#212139PurposeCargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertxylitol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT03
Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynCAM4/pCDNA3
Plasmid#197328PurposePlasmid expressing an antigen targeting NECL4/SynCAM4DepositorInsertSynCAM4 (Cadm4 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
