We narrowed to 1,369 results for: SAG

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc0-malat1

Plasmid

#210580

Purpose

Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-malat1

Plasmid

#210581

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1_Short_V5-GFP

Plasmid

#221500

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_CdTag-HA

Plasmid

#221493

Purpose

NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 224-371AA C-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

hPGK

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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G1088E_pLenti-CMV-mNeonGreen-2A-HygroR

Plasmid

#216279

Purpose

Green and hygromycin resistant lentiviral vector plasmid for multiplexed receptor pseudovirus experiments

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2024 May 20;20(5):e1012044.

Insert

mNeonGreen-2A-Hpt

Use

Lentiviral

Promoter

CMV

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_L172Q_KDEL_pBABEpu

Plasmid

#183690

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(L172Q)

Use

Retroviral

Mutation

L72Q in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

Plasmid

#183691

Purpose

Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(M21K_F86L)

Use

Retroviral

Mutation

M21K_F86L in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_Jdomain

Plasmid

#183693

Purpose

Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)-J domain

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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MLSE-shTAF12.376

Plasmid

#105578

Purpose

retrovirally express TAF12 shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF12 shRNA #376

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTADA1.1580

Plasmid

#105568

Purpose

retrovirally express TADA1 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTADA1.1226

Plasmid

#105567

Purpose

retrovirally express TADA1 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TADA1 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LEP-shTAF4.3818

Plasmid

#105564

Purpose

retrovirally express TAF4 shRNA with puro resistance and GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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