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35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-VP16/pAM
Plasmid#201232PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-GWY-VP16
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…PromoterAvailable sinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralTagsExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-RA
Plasmid#209872PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddRFP A
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon Nega
Plasmid#163117PurposeRed fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cellsDepositorInsertRed Glifon Nega
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ER-B
Plasmid#209870PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP B
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mito-GA
Plasmid#209865PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the mitochondrial membrane with C-terminal targeting sequence of human MAVSDepositorInsertddGFP A
UseTagsTargeting domain of MAVS RPSPGALWLQVAVTGVLVVTLLVV…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-NLS-mTagBFP2
Plasmid#216128PurposeLentiviral vector for expressing mTagBFP2 localizing to the nucleusDepositorInsertEF1a-NuclearLocalizationSignal-mTagBFP2-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
UseTagstdTomato-v5-GCaMP6fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-col4a1
Plasmid#160317PurposecfSGFP2-tagged collagen4a1 for mammalian expressionDepositorInsertCOL4A1 (COL4A1 Human)
UseTagscfSGFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-iRFP713-ORP9-PH
Plasmid#214269PurposeMammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PHDepositorInsertiRFP713-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiRhoA2G
Plasmid#40179DepositorInsertRhoA Biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1-T2A-tdTomato
Plasmid#60598PurposeExpresses Kir2.1-T2A-tdTomatoDepositorInsertKir2.1-T2A-tdTomato (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only
