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Plasmid#173455PurposeContains mScarlet-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFA104.PI.eGFP.WPRE.bGH
Plasmid#100896PurposeAAV expression of EGFP in astrocytesDepositorHas ServiceAAV5InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterGFA104 (GFAP promoter)Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
plvx-tetO-GFP
Plasmid#191560PurposeInducible expression of GFPDepositorInsertgfp
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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HI.fate.SFFV-E
Plasmid#191555PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.E
Plasmid#191553PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.UB
Plasmid#191556PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HI.fate.SFFV
Plasmid#191554PurposeMonitor HIV-1 fateDepositorInsertHIV-1 incomplete genome
UseLentiviralTagsExpressionMutationenv-deleted + vpr mutation(s)PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TCF12
Plasmid#67113PurposeStandard for cell-free expression benchmarking.DepositorInsertTCF12 (TCF12 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Endoplasmic reticulum-targeted optoPLD
Plasmid#140060PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sac1(TM), and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA
Plasmid#84277PurposepLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter.DepositorInsertGluN2B
UseLentiviralTagsGFPExpressionMutationPromoterCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA
Plasmid#84276PurposepLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter.DepositorInsertGluN2A
UseLentiviralTagsGFPExpressionMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optoPLD
Plasmid#140056PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
UseTags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03
Plasmid#67137PurposeGateway destination vector for cell-free expression with N-term 8xHis eGFP tagDepositorTypeEmpty backboneUseCell-free expressionTagsEGFP and HisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-cGAS g2 Cas9-T2A-mCherry
Plasmid#164253PurposeTranscription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgcGAS
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2A13
Plasmid#67103PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2A13 (CYP2A13 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-3xFRB
Plasmid#20223DepositorInsert3xFRB (MTOR Human)
UseTagsHA and VhExpressionMammalianMutationOne copy of FRB has a T2098L mutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pU6-cGAS g3 Cas9-T2A-mCherry
Plasmid#164254PurposeTranscription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgcGAS
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-24XGP41-J9
Plasmid#202006PurposeMoClo level 0 dCas9-24XGP41 coding sequence moduleDepositorInsertdCas9-24XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersExpressionMutationPromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only