We narrowed to 6,871 results for: addgene/1000

1. pGEX6P1-merlin 1-339

   Plasmid

   #168128

   Purpose

   Bacterial expression of human Nf2/merlin 1-339

   Depositor

   Tina Izard

   Article

   Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

   Insert

   Merlin (NF2 Human)
   

   Use

   Tags

   Glutathione S-transferase (GST)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-HsProm1s1(W795R)-StayGold

   Plasmid

   #210822

   Purpose

   Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   StayGold

   Expression

   Mammalian

   Mutation

   Changed Trp-795 to Arg

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-HsProm1s1-TwinStrep-His

   Plasmid

   #210816

   Purpose

   Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK-ENH_BsmB1-Avi-MCS-2A-Citrine

   Plasmid

   #110204

   Purpose

   Recipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Lukoseviciute et al STAR Protoc. 2020 Jul 31;1(2):100066. do

   Insert

   ENH_BsmB1-Avi-MCS-2A-Citrine
   

   Use

   Tags

   Avi_tag-2a-YFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YF

   Plasmid

   #171459

   Purpose

   Expresses YF, a FiCE of iCMM, in mammalian cells

   Depositor

   Takafumi Miyamoto

   Article

   Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

   Insert

   EYFP-FKBP12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAVS1-Puro CAG J591-scFV T-CAR

   Plasmid

   #171961

   Purpose

   Donor plasmid for J591 T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic J591-scFV CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro CAG PSMA nanobody T-CAR

   Plasmid

   #171962

   Purpose

   Donor plasmid for PSMA nanobody T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic anti-PSMA nanobody CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB2-neo-TET

   Plasmid

   #51287

   Purpose

   expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   sine B2
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   enhancer from 7SL gene

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAluYa5-neo-TET

   Plasmid

   #51283

   Purpose

   expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   AluYa5
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   upstream pol III enhancer sequence of the 7SL gen…

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKIIa-mScarlet

    Plasmid

    #131000

    Purpose

    AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5233

    Plasmid

    #128715

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcM-hopM1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

    Plasmid

    #159283

    Purpose

    A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Inserts

    Human INS sgRNA
    Human INS guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHS1861

    Plasmid

    #205974

    Purpose

    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHS1871

    Plasmid

    #205976

    Purpose

    U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH DR-BpiI-DR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS1517

    Plasmid

    #205980

    Purpose

    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHS1929

    Plasmid

    #205968

    Purpose

    JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1477

    Plasmid

    #205970

    Purpose

    MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH all proteins, with His-tagged Cas11
    

    Use

    Tags

    14xHis on Cas11

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS-HsProm1s1(W795R)-StayGold

    Plasmid

    #211334

    Purpose

    Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    AAV

    Tags

    StayGold

    Expression

    Mutation

    Changed Trp-795 to Arg

    Promoter

    EF1a

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-HsProm1s1(W795R)-TwinStrep-His

    Plasmid

    #210817

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Changed Trp-795 to Arg

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Src(KD/YF)-SsrA(Y7I)-mCer3

    Plasmid

    #221000

    Purpose

    this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA, mCerulean3 (Src Mouse)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D388R/Y529F in Src, Y7I in SsrA

    Promoter

    pCMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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