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We narrowed to 4,378 results for: crispr c plasmids

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Showing: 621 - 640 of 4378 results

  1. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227307-plasmid-map-sequence-id-451546

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  3. pJAT25

    Plasmid
    #204310
    Purpose
    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-GFPc
    Use
    CRISPR
    Promoter
    ie1
    Available Since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204310-plasmid-map-sequence-id-405854

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  4. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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  14. AICSDP-55:DMD-mEGFP

    Plasmid
    #159744
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159744-plasmid-map-sequence-id-314374

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  15. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

    Plasmid
    #139995
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    HF1=N497A/R661A/Q695A/Q926A
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139995-plasmid-map-sequence-id-275425

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  18. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140000-plasmid-map-sequence-id-275479

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  20. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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Showing: 621 - 640 of 4378 results